Anti-PKC antibody [MC5] (ab31)
![Anti-PKC antibody [MC5] (ab31)](https://www.abcam.com/ps/products/0/ab31/Images/ab31-3209-anti-pkc-antibody-mc5-western-blot.jpg "Anti-PKC antibody [MC5] (ab31)")
Key features and details
- Mouse monoclonal [MC5] to PKC
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Rat, Human
- Isotype: IgG2a
Overview
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Product name
Anti-PKC antibody [MC5]
See all PKC primary antibodies -
Description
Mouse monoclonal [MC5] to PKC -
Host species
Mouse -
Specificity
This antibody reacts with PKC alpha, crossreactivity to PKC beta isoformes can be observed. -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human
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Immunogen
Purified bovine brain protein kinase C
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Positive control
- WB: Calpain treated PKC. Rat adipocyte homogenate. 3T3-L1 homogenate. Purified microsomes from 3T3-L1 cells. Flow Cytometry: Jurkat cells. ICC/IF: Vero and HEK-293T cells. IHC-Fr: mouse spleen and duodenum tissue. IHC-P: Human skin papilloma tissue.
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General notes
It should be possible to selectively block degradation of PKC without affecting the membrane associated activation. This will allow an assessment of the role of proteolysis in the activation of the protein kinase pathway.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
MC5 -
Myeloma
P3x63 -
Isotype
IgG2a -
Light chain type
unknown -
Research areas
Images
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Western blot - Anti-PKC antibody [MC5] (ab31)All lanes : Anti-PKC antibody [MC5] (ab31)
Lane 1 : Calpain treated PKC
Lane 2 : Homogenate from rat adipocytes
Lane 3 : Homogenate from 3T3-L1 cells
Lane 4 : Purified microsomes from 3T3-L1 cellsReview by Mikael Rutberg submitted 2 September 2004.
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![Flow Cytometry - Anti-PKC antibody [MC5] (ab31)](https://www.abcam.com/ps/products/0/ab31/Images/ab31-195386-anti-pkc-antibody-mc5-flow-cytometry.jpg)
Flow Cytometry - Anti-PKC antibody [MC5] (ab31)Overlay histogram showing Jurkat (Human T cell leukemia cell line from peripheral blood) cells stained with ab31 (red line). The cells were fixed with 80% methanol (5 minutes) and then permeabilized with 0.1% PBS-Tween for 20 minutes. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab31, 1 μg/1x106 cells) for 30 minutes at 22°C. The secondary antibody used was a goat anti-mouse Alexa Fluor® 488 (IgG; H+L) ab150113 at 1/2000 dilution for 30 minutes at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1 μg/1x106 cells) used under the same conditions. Unlabeled sample (blue line) was used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
This antibody gave a positive signal in HL-60 (Human promyelocytic leukemia cell line) cells fixed with 4% paraformaldehyde (10 minutes)/permeabilized with 0.1% PBS-Tween for 20 minutes used under the same conditions.
