Anti-MARCKS (phospho S158) antibody [EP2113Y] (ab81295)
![Anti-MARCKS (phospho S158) antibody [EP2113Y] (ab81295)](https://www.abcam.com/ps/products/81/ab81295/Images/ab81295-239710-anti-marcks-phospho-s158-antibody-ep2113y-western-blot.jpg "Anti-MARCKS (phospho S158) antibody [EP2113Y] (ab81295)")
Key features and details
- Rabbit monoclonal [EP2113Y] to MARCKS (phospho S158)
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-MARCKS (phospho S158) antibody [EP2113Y]
See all MARCKS primary antibodies -
Description
Rabbit monoclonal [EP2113Y] to MARCKS (phospho S158) -
Host species
Rabbit -
Specificity
This antibody only detects MARCKS phosphorylated at serine 158. -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human MARCKS. The exact sequence is proprietary.
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Positive control
- Hela cell lysates treated with TPA.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP2113Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MARCKS (phospho S158) antibody [EP2113Y] (ab81295)All lanes : Anti-MARCKS (phospho S158) antibody [EP2113Y] (ab81295) at 1/2000 dilution (purified)
Lane 1 : Rat brain
Lane 2 : Mouse brain
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 32 kDa
Observed band size: 87 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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![Western blot - Anti-MARCKS (phospho S158) antibody [EP2113Y] (ab81295)](https://www.abcam.com/ps/products/81/ab81295/Images/ab81295-239709-anti-marcks-phospho-s158-antibody-ep2113y-western-blot.jpg)
Western blot - Anti-MARCKS (phospho S158) antibody [EP2113Y] (ab81295)All lanes : Anti-MARCKS (phospho S158) antibody [EP2113Y] (ab81295) at 1/10000 dilution (purified)
Lane 1 : HEK293 cells treated with TPA
Lane 2 : HEK293 cells treated with TPA, and the PVDF membrane treated with lambda phosphatase 10ug
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 32 kDa
Observed band size: 87 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
-
![Western blot - Anti-MARCKS (phospho S158) antibody [EP2113Y] (ab81295)](https://www.abcam.com/ps/products/81/ab81295/Images/ab81295-96500-anti-marcks-phospho-s158-antibody-ep2113y-western-blot.jpg)
Western blot - Anti-MARCKS (phospho S158) antibody [EP2113Y] (ab81295)All lanes : Anti-MARCKS (phospho S158) antibody [EP2113Y] (ab81295) at 1/2000 dilution (unpurified)
Lane 1 : Hela cell lysates, untreated.
Lane 2 : Hela cell lysates treated with TPA.
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti rabbit at 1/2000 dilution
Predicted band size: 32 kDa
Observed band size: 87 kDa why is the actual band size different from the predicted?
