Anti-PKC zeta antibody (ab59364)
Key features and details
- Rabbit polyclonal to PKC zeta
- Suitable for: ELISA, IHC-P, IP, WB, ICC/IF
- Reacts with: Mouse, Rat, Human, Xenopus laevis, African green monkey
- Isotype: IgG
Overview
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Product name
Anti-PKC zeta antibody
See all PKC zeta primary antibodies -
Description
Rabbit polyclonal to PKC zeta -
Host species
Rabbit -
Specificity
ab59364 detects endogenous levels of total PKC zeta protein. The immunogen used for this product shares 89% homology with PKC iota/lambda. Cross-reactivity with this protein has not been confirmed experimentally. -
Tested applications
Suitable for: ELISA, IHC-P, IP, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Xenopus laevis, African green monkey -
Immunogen
Synthetic peptide within Human PKC zeta aa 526-575. The exact sequence is proprietary. Synthesized non-phosphopeptide derived from human PKC zeta around the phosphorylation site of threonine 560 (Q-L-TP-P-D).
Database link: Q05513 -
Positive control
- IHC-P: Human proliferative endometrial tissue, human brain tissue. ICC: NIH/3T3 cells. WB: Rat hippocampus lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PKC zeta antibody (ab59364) Verified CustomerAnti-PKC zeta antibody (ab59364) at 1/500 dilution + Xenopus laevis cytoplasmic egg extract at 40 µg
Secondary
Goat polyclonal - IRDye® 800CW at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 68 kDa
Exposure time: 5 seconds
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC zeta antibody (ab59364) Image from Rivero R et al., Reproductive Biology and Endocrinology 2012, 10:17, 5 March 2012. Fig 1.; doi: 10.1186/1477-7827-10-17.Immunohistochemical analysis of human proliferative endometrial tissue, staining PKC zeta with ab59364.
Antigen retrieval was performed by heat mediation in a citrate buffer. Tissue was blocked with blocking solution before incubating with primary antibody (1/100) overnight at 4°C. Staining was detected using DAB. -
Immunocytochemistry/ Immunofluorescence - Anti-PKC zeta antibody (ab59364)Immunofluorescence analysis of NIH/3T3 cells using PKC zeta at dilution of 1:100.
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Western blot - Anti-PKC zeta antibody (ab59364)All lanes : Anti-PKC zeta antibody (ab59364) at 1/500 dilution
Lane 1 : Extracts from COS-7 (African green monkey kidney fibroblast-like cell line) cells, treated with PMA (125 ng/ml, 30mins), without immunizing peptide
Lane 2 : Extracts from COS7 (African green monkey kidney fibroblast-like cell line)cells, treated with PMA (125 ng/ml, 30mins), plus immunizing peptide
Predicted band size: 68 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
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Western blot - Anti-PKC zeta antibody (ab59364) This image is courtesy of an Abreview submitted by Mr Richard D'MelloLanes 2-4 : Anti-PKC zeta antibody (ab59364) at 1/500 dilution
Lanes 1-2 : Rat hippocampus lysate at 40 µg
Lanes 3-4 : Rat hippocampus lysate at 20 µg
Secondary
All lanes : Anti-Rabbit IR800-linked conjugation: IRDye® 800CW
Performed under reducing conditions.
Predicted band size: 68 kDa
Exposure time: 5 minutes
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC zeta antibody (ab59364)ab59364 at a 1/50 dilution staining PKC zeta in formalin/PFA fixed paraffin-embedded human brain tissue using Immunohistochemistry.
Left panel: Without immunizing peptide.
Right image: Plus immunizing peptide.
