Anti-PKC theta/PRKCQ antibody [M217] (ab76197)
![Anti-PKC theta/PRKCQ antibody [M217] (ab76197)](https://www.abcam.com/ps/products/76/ab76197/Images/ab76197-91555-anti-pkc-theta-antibody-m217-western-blot.gif "Anti-PKC theta/PRKCQ antibody [M217] (ab76197)")
Key features and details
- Mouse monoclonal [M217] to PKC theta/PRKCQ
- Suitable for: ICC/IF, WB
- Reacts with: Rat, Human
- Isotype: IgG2a
Overview
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Product name
Anti-PKC theta/PRKCQ antibody [M217]
See all PKC theta/PRKCQ primary antibodies -
Description
Mouse monoclonal [M217] to PKC theta/PRKCQ -
Host species
Mouse -
Specificity
ab76197 is not predicted to react with other PKC family members due to low sequence homology. -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Jurkat whole cell lysate (ab7899). ICC/IF: PC-12 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 50% Glycerol, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
M217 -
Isotype
IgG2a -
Research areas
Images
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Western blot - Anti-PKC theta/PRKCQ antibody [M217] (ab76197)Lane 1 : Anti-PKC theta/PRKCQ antibody [M217] (ab76197) at 1/250 dilution
Lane 2 : Anti-PKC theta/PRKCQ antibody [M217] (ab76197) at 1/1000 dilution
Lane 1 : Jurkat cell lysate.
Lane 2 : Jurkat cell lysate
Predicted band size: 82 kDa
Observed band size: 79 kDa why is the actual band size different from the predicted?
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![Immunocytochemistry/ Immunofluorescence - Anti-PKC theta/PRKCQ antibody [M217] (ab76197)](https://www.abcam.com/ps/products/76/ab76197/Images/ab76197-351583-anti-pkc-theta-prkcq-antibody-m217-immunocytochemistry-pc12-rat.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PKC theta/PRKCQ antibody [M217] (ab76197)NGF-differentiated PC-12 cells (rat adrenal gland pheochromocytoma cell line) stained for PKC theta/PRKCQ using ab76197 at 1/100 dilution in ICC/IF. Secondary antibody is a Cy3 conjugate.
