Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IKK alpha antibody [EPR464] - BSA and Azide free (ab171362)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR464] to IKK alpha - BSA and Azide free
  • Suitable for: ICC, IHC-P, IP, WB, Flow Cyt
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-IKK alpha antibody [EPR464] - BSA and Azide free
    See all IKK alpha primary antibodies

  • Description

    Rabbit monoclonal [EPR464] to IKK alpha - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, IHC-P, IP, WB, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab171362 is the carrier-free version of ab109749 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab171362 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-IKK alpha antibody [EPR464] - BSA and Azide free (ab171362)
    Western blot - Anti-IKK alpha antibody [EPR464] - BSA and Azide free (ab171362)

    This data was developed using ab109749, the same antibody clone in a different buffer formulation.

    Lanes 1, 3, and 5:ild-type HAP1 cell lysate (20 µg)

    Lanes 2, 4, and 6: IKK alpha knockout HAP1 cell lysate (20 µg)

    Lanes 1 and 2: Green signal from target - ab109749 observed at 84 kDa

    Lanes 3 and 4: Red signal from loading control - ab8226 observed at 42 kDa

    Lanes 5 and 6: Merged (red and green) signal

    ab109749 was shown to specifically react with IKK alpha when IKK alpha knockout samples were used. Wild-type and IKK alpha knockout samples were subjected to SDS-PAGE. ab109749 and ab8226 (loading control to beta actin) were both diluted at 1/1000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776)secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

  • Immunocytochemistry - Anti-IKK alpha antibody [EPR464] - BSA and Azide free (ab171362)
    Immunocytochemistry - Anti-IKK alpha antibody [EPR464] - BSA and Azide free (ab171362)
    This data was developed using ab109749, the same antibody clone in a different buffer formulation.Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labeling IKK alpha with purified ab109749 at 1/250 dilution. Cells were fixed with 4% PFA and permeabilized with 0.1% tritonX-100. ab150077 Goat anti rabbit IgG (Alexa Fluor®488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.
  • Flow Cytometry - Anti-IKK alpha antibody [EPR464] - BSA and Azide free (ab171362)
    Flow Cytometry - Anti-IKK alpha antibody [EPR464] - BSA and Azide free (ab171362)
    This data was developed using ab109749, the same antibody clone in a different buffer formulation.Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling IKK alpha with unpurified ab109749 at 1/50 dilutio n(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
  • Western blot - Anti-IKK alpha antibody [EPR464] - BSA and Azide free (ab171362)
    Western blot - Anti-IKK alpha antibody [EPR464] - BSA and Azide free (ab171362)
    All lanes : Anti-IKK alpha antibody [EPR464] (ab109749) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : Daudi cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 85 kDa



    This data was developed using ab109749, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKK alpha antibody [EPR464] - BSA and Azide free (ab171362)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKK alpha antibody [EPR464] - BSA and Azide free (ab171362)
    This data was developed using ab109749, the same antibody clone in a different buffer formulation.ab109749, at 1/100 dilution, staining IKK alpha in paraffin-embedded Human breast tissue by Immunohistochemistry. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Anti-IKK alpha antibody [EPR464] - BSA and Azide free (ab171362)
    Anti-IKK alpha antibody [EPR464] - BSA and Azide free (ab171362)

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