Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Loricrin antibody [EPR7149(2)] - BSA and Azide free (ab240345)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7149(2)] to Loricrin - BSA and Azide free
  • Suitable for: ICC/IF, IHC-P
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-Loricrin antibody [EPR7149(2)] - BSA and Azide free
    See all Loricrin primary antibodies

  • Description

    Rabbit monoclonal [EPR7149(2)] to Loricrin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details

  • Species reactivity

    Reacts with: Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab240345 is the carrier-free version of ab198994.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Loricrin antibody [EPR7149(2)] - BSA and Azide free (ab240345)
    Immunocytochemistry/ Immunofluorescence - Anti-Loricrin antibody [EPR7149(2)] - BSA and Azide free (ab240345)

    Immunocytochemistry/ Immunofluorescence analysis of A431 cell line labeling Loricrin with ab198994 at a 1/250 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized using 0.1% Triton X-100. Alexa Fluor® 488 Goat anti-Rabbit ab150077 used as the secondary at a 1/1000 dilution. Counterstained using ab7291 anti-Tubulin (mouse mAb) at a 1/1000 dilution and ab150120 Alexa Fluor® 594 Goat anti-Mouse secondary at a 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198994).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Loricrin antibody [EPR7149(2)] - BSA and Azide free (ab240345)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Loricrin antibody [EPR7149(2)] - BSA and Azide free (ab240345)

    Immunohistochemical analysis of paraffin-embedded rat skin tissue sections labeling Loricrin with ab198994 at a 1/500 dilution. Goat anti-rabbit IgG H&L (HRP) ab97051 used as the secondary at a 1/500 dilution. Counterstain hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198994).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Loricrin antibody [EPR7149(2)] - BSA and Azide free (ab240345)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Loricrin antibody [EPR7149(2)] - BSA and Azide free (ab240345)

    Immunohistochemical analysis of paraffin-embedded human uterus tissue sections labeling Loricrin with ab198994 at a 1/500 dilution. Goat anti-rabbit IgG H&L (HRP) ab97051 used as the secondary at a 1/500 dilution. Counterstain hematoxylin. Used as a negative tissue control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198994).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Loricrin antibody [EPR7149(2)] - BSA and Azide free (ab240345)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Loricrin antibody [EPR7149(2)] - BSA and Azide free (ab240345)

    Immunohistochemical analysis of paraffin-embedded human skin tissue sections labeling Loricrin with ab198994 at a 1/500 dilution. Goat anti-rabbit IgG H&L (HRP) ab97051 used as the secondary at a 1/500 dilution. Counterstain hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198994).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-Loricrin antibody [EPR7149(2)] - BSA and Azide free (ab240345)
    Anti-Loricrin antibody [EPR7149(2)] - BSA and Azide free (ab240345)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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