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Anti-DCK antibody [OTI16G6] - BSA and Azide free (ab273650)

Key features and details

  • Mouse monoclonal [OTI16G6] to DCK - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-DCK antibody [OTI16G6] - BSA and Azide free
    See all DCK primary antibodies

  • Description

    Mouse monoclonal [OTI16G6] to DCK - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant full length protein corresponding to Human DCK aa 1-300.
    Database link: P27707

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: pCMV6-ENTRY DCK transfected HEK-293T lysate; HepG2, HeLa, Jurkat cell lysate, human testis, omentum, uterus, breast, brain, liver, ovary, thyroid gland and colon tissue lysate; IHC-P: Human breast adenocarcinoma, lung carcinoma, bladder carcinoma, lymph node, lymphoma tissue; Flow Cyt (Intra): HeLa and Jurkat whole cells.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Images

  • Western blot - Anti-DCK antibody [OTI16G6] - BSA and Azide free (ab273650)
    Western blot - Anti-DCK antibody [OTI16G6] - BSA and Azide free (ab273650)
    All lanes : Anti-DCK antibody [OTI16G6] - BSA and Azide free (ab273650) at 1/2000 dilution

    Lane 1 : Human testis tissue lysate
    Lane 2 : Human omentum tissue lysate
    Lane 3 : Human uteus tissue lysate
    Lane 4 : Human breast tissue lysate
    Lane 5 : Human brain tissue lysate
    Lane 6 : Human liver tissue lysate
    Lane 7 : Human ovary tissue lysate
    Lane 8 : Human thyroid tissue lysate
    Lane 9 : Human colon tissue lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 31 kDa



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCK antibody [OTI16G6] - BSA and Azide free (ab273650)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCK antibody [OTI16G6] - BSA and Azide free (ab273650)

    Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue staining DCK with a mouse monoclonal
    DCK antibody [OTI16G6].

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

  • Flow Cytometry - Anti-DCK antibody [OTI16G6] - BSA and Azide free (ab273650)
    Flow Cytometry - Anti-DCK antibody [OTI16G6] - BSA and Azide free (ab273650)

    Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) whole cell using a mouse monoclonal DCK antibody [OTI16G6] (Red), compared to a nonspecific negative control antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCK antibody [OTI16G6] - BSA and Azide free (ab273650)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCK antibody [OTI16G6] - BSA and Azide free (ab273650)

    Immunohistochemical analysis of paraffin-embedded human breast adenocarcinoma tissue staining DCK with a mouse monoclonal
    DCK antibody [OTI16G6]. 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

  • Western blot - Anti-DCK antibody [OTI16G6] - BSA and Azide free (ab273650)
    Western blot - Anti-DCK antibody [OTI16G6] - BSA and Azide free (ab273650)
    All lanes : Anti-DCK antibody [OTI16G6] - BSA and Azide free (ab273650) at 1/2000 dilution

    Lane 1 : pCMV6-ENTRY control-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : pCMV6-ENTRY DCK-transfected HEK-293T whole cell lysate

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 31 kDa



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

  • Flow Cytometry - Anti-DCK antibody [OTI16G6] - BSA and Azide free (ab273650)
    Flow Cytometry - Anti-DCK antibody [OTI16G6] - BSA and Azide free (ab273650)

    Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate using a mouse monoclonal DCK antibody [OTI16G6] (Red), compared to a nonspecific negative control antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCK antibody [OTI16G6] - BSA and Azide free (ab273650)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCK antibody [OTI16G6] - BSA and Azide free (ab273650)

    Immunohistochemical analysis of paraffin-embedded human bladder carcinoma tissue staining DCK with a mouse monoclonal
    DCK antibody [OTI16G6]. 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCK antibody [OTI16G6] - BSA and Azide free (ab273650)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCK antibody [OTI16G6] - BSA and Azide free (ab273650)

    Immunohistochemical analysis of paraffin-embedded human lymph node tissue staining DCK with a mouse monoclonal
    DCK antibody [OTI16G6]. 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCK antibody [OTI16G6] - BSA and Azide free (ab273650)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCK antibody [OTI16G6] - BSA and Azide free (ab273650)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue staining DCK with a mouse monoclonal
    DCK antibody [OTI16G6]. 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

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