Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ASH2L antibody [EPR13107(B)] - BSA and Azide free (ab240191)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13107(B)] to ASH2L - BSA and Azide free
  • Suitable for: WB, ICC/IF, IHC-P, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ASH2L antibody [EPR13107(B)] - BSA and Azide free
    See all ASH2L primary antibodies

  • Description

    Rabbit monoclonal [EPR13107(B)] to ASH2L - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, IHC-P, Flow Cytmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, 293T, Jurkat and K562 cell lysates. IHC-P: Human colon, breast and testis tissues, mouse liver, and rat cerebrum tissues. ICC/IF: HeLa and 293T cells. Flow Cyt: HeLa cells.

  • General notes

    ab240191 is the carrier-free version of ab176334. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab240191 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-ASH2L antibody [EPR13107(B)] - BSA and Azide free (ab240191)
    Immunocytochemistry/ Immunofluorescence - Anti-ASH2L antibody [EPR13107(B)] - BSA and Azide free (ab240191)

    Immunocytochemistry/ Immunofluorescence analysis of 293T (Human embryonic kidney epithelial cell) cells labeling ASH2L with purified ab176334 at 1/50 dilution (7.78 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176334).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASH2L antibody [EPR13107(B)] - BSA and Azide free (ab240191)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASH2L antibody [EPR13107(B)] - BSA and Azide free (ab240191)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebrum tissue sections labeling ASH2L with purified ab176334 at 1/8000 dilution (0.05 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176334).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASH2L antibody [EPR13107(B)] - BSA and Azide free (ab240191)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASH2L antibody [EPR13107(B)] - BSA and Azide free (ab240191)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue sections labeling ASH2L with purified ab176334 at 1/8000 dilution (0.05 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176334).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASH2L antibody [EPR13107(B)] - BSA and Azide free (ab240191)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASH2L antibody [EPR13107(B)] - BSA and Azide free (ab240191)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast tissue sections labeling ASH2L with purified ab176334 at 1/8000 dilution (0.05 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176334).

  • Flow Cytometry - Anti-ASH2L antibody [EPR13107(B)] - BSA and Azide free (ab240191)
    Flow Cytometry - Anti-ASH2L antibody [EPR13107(B)] - BSA and Azide free (ab240191)

    Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling ASH2L (red) with ab176334 at a 1/1200 dilution. Cells were fixed with 80% methanol and permeabilized with 0.1% Tween-20. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176334).

  • Immunocytochemistry/ Immunofluorescence - Anti-ASH2L antibody [EPR13107(B)] - BSA and Azide free (ab240191)
    Immunocytochemistry/ Immunofluorescence - Anti-ASH2L antibody [EPR13107(B)] - BSA and Azide free (ab240191)

    Immunofluorescent analysis of HeLa labeling ASH2Lwith ab176334 at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176334).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASH2L antibody [EPR13107(B)] - BSA and Azide free (ab240191)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASH2L antibody [EPR13107(B)] - BSA and Azide free (ab240191)

    Immunohistochemical analysis of paraffin-embedded Human testis tissue labeing ASH2L with ab176334 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176334).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASH2L antibody [EPR13107(B)] - BSA and Azide free (ab240191)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASH2L antibody [EPR13107(B)] - BSA and Azide free (ab240191)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeing ASH2L with ab176334 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176334).

  • Anti-ASH2L antibody [EPR13107(B)] - BSA and Azide free (ab240191)
    Anti-ASH2L antibody [EPR13107(B)] - BSA and Azide free (ab240191)

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