Antibodies, Proteins, Kits and Reagents for Life Science

311 587 ₸ Product size

100 µl 311 587 ₸ 10 µl 127 315 ₸

Order now and get it on Tuesday March 09, 2021

Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker (ab176334)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR13107(B)] to ASH2L - Nuclear Marker
Suitable for: ICC/IF, Flow Cyt, IHC-P, WB
Reacts with: Mouse, Rat, Human

Product name

Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker
See all ASH2L primary antibodies
Description

Rabbit monoclonal [EPR13107(B)] to ASH2L - Nuclear Marker
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Mouse Rat Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide within Human ASH2L aa 600 to the C-terminus (C terminal) (Cysteine residue). The exact sequence is proprietary.
Database link: Q9UBL3
Positive control
- WB: HeLa, 293T, Jurkat and K562 cell lysates. IHC-P: Human colon, breast and testis tissues, mouse liver, and rat cerebrum tissues. ICC/IF: HeLa and 293T cells. Flow Cyt: HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR13107(B)
Isotype

IgG
Research areas

Western blot - Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker (ab176334)

All lanes : Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker (ab176334) at 1/10000 dilution (Purified)

Lane 1 : 293T (Human embryonic kidney epithelial cell) whole cell lysates
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 3 : Mouse heart lysates
Lane 4 : Rat heart lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 69 kDa
Observed band size: 65,80 kDa
why is the actual band size different from the predicted?

ab176334 recoginzes 3 isoforms of ASH2L
Immunocytochemistry/ Immunofluorescence - Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker (ab176334)

Immunocytochemistry/ Immunofluorescence analysis of 293T (Human embryonic kidney epithelial cell) cells labeling ASH2L with purified ab176334 at 1/50 dilution (7.78 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker (ab176334)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebrum tissue sections labeling ASH2L with purified ab176334 at 1/8000 dilution (0.05 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker (ab176334)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue sections labeling ASH2L with purified ab176334 at 1/8000 dilution (0.05 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker (ab176334)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast tissue sections labeling ASH2L with purified ab176334 at 1/8000 dilution (0.05 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Western blot - Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker (ab176334)

All lanes : Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker (ab176334) at 1/10000 dilution ((unpurified))

Lane 1 : HeLa cell lysates
Lane 2 : 293T cell lysates
Lane 3 : Jurkat cell lysates
Lane 4 : K562 cell lysates

Lysates/proteins at 10 µg per lane.

Predicted band size: 69 kDa
Immunocytochemistry/ Immunofluorescence - Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker (ab176334)

Immunofluorescent analysis of HeLa labeling ASH2L with ab176334 at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker (ab176334)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeing ASH2L with unpurified ab176334 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cytometry - Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker (ab176334)

Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling ASH2L (red) with unpurified ab176334 at a 1/1200 dilution. Cells were fixed with 80% methanol and permeabilized with 0.1% Tween-20. A goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker (ab176334)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeing ASH2L with unpurified ab176334 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker (ab176334)

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