Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HDAC8 antibody [EPR23837-45] (ab274372)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23837-45] to HDAC8
  • Suitable for: IP, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-HDAC8 antibody [EPR23837-45]
    See all HDAC8 primary antibodies

  • Description

    Rabbit monoclonal [EPR23837-45] to HDAC8

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Mouse
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HAP1, K562, HeLa,LADMAC and C6 whole cell lysates; Mouse, human and rat brain tissue lysate. IP: K562 and NIH/3T3 whole cell lysates.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-HDAC8 antibody [EPR23837-45] (ab274372)
    Western blot - Anti-HDAC8 antibody [EPR23837-45] (ab274372)
    All lanes : Anti-HDAC8 antibody [EPR23837-45] (ab274372) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate at 20 µg
    Lane 2 : HDAC8 knockout HAP1 whole cell lysate at 20 µg
    Lane 3 : Mouse brain tissue lysate
    Lane 4 : Rat brain tissue lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution

    Predicted band size: 42 kDa
    Observed band size: 42 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Ab274372 was shown to specifically react with HDAC8 in wild-type HAP1 cells as signal was lost in HDAC8 knockout cells. Wild-type and HDAC8 knockout samples were subjected to SDS-PAGE. ab274372 and ab129002 (Rabbit anti-Vinculin loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/5000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/50, 000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™MP instrument using the ECL technique.

    Exposure time: 3 minutes

     

  • Immunoprecipitation - Anti-HDAC8 antibody [EPR23837-45] (ab274372)
    Immunoprecipitation - Anti-HDAC8 antibody [EPR23837-45] (ab274372)

    HDAC8 was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate with ab274372 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab274372 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 10 ug.

    Lane 2: ab274372 IP in NIH/3T3 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab274372 in NIH/3T3 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

     

  • Western blot - Anti-HDAC8 antibody [EPR23837-45] (ab274372)
    Western blot - Anti-HDAC8 antibody [EPR23837-45] (ab274372)
    Anti-HDAC8 antibody [EPR23837-45] (ab274372) at 1/1000 dilution + Human brain tissue lysate at 20 µg

    Secondary
    VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution

    Predicted band size: 42 kDa
    Observed band size: 42 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This blot was developed using a higher sensitivity ECL substrate.

    Exposure time: 3 minutes

     

  • Western blot - Anti-HDAC8 antibody [EPR23837-45] (ab274372)
    Western blot - Anti-HDAC8 antibody [EPR23837-45] (ab274372)
    All lanes : Anti-HDAC8 antibody [EPR23837-45] (ab274372) at 1/1000 dilution

    Lane 1 : K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate
    Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 3 : LADMAC (mouse bone marrow monocyte macrophage) whole cell lysate
    Lane 4 : C6 (rat glial tumor glial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution

    Predicted band size: 42 kDa
    Observed band size: 42 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

     

  • Immunoprecipitation - Anti-HDAC8 antibody [EPR23837-45] (ab274372)
    Immunoprecipitation - Anti-HDAC8 antibody [EPR23837-45] (ab274372)

    HDAC8 was immunoprecipitated from 0.35 mg K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate with ab274372 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab274372 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate 10 ug.

    Lane 2: ab274372 IP in K-562 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab274372 in K-562 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

     

  • Anti-HDAC8 antibody [EPR23837-45] (ab274372)
    Anti-HDAC8 antibody [EPR23837-45] (ab274372)

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