Antibodies, Proteins, Kits and Reagents for Life Science

271 382 ₸ Product size

100 µl 271 382 ₸

Order now and get it on Tuesday March 09, 2021

Anti-LGALS14 antibody [EPR9665] (ab150427)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR9665] to LGALS14
Suitable for: WB, ICC/IF, Flow Cyt
Reacts with: Mouse, Human

Product name

Anti-LGALS14 antibody [EPR9665]
See all LGALS14 primary antibodies
Description

Rabbit monoclonal [EPR9665] to LGALS14
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
WB	Mouse Human

See all applications and species data

Immunogen

Synthetic peptide within Human LGALS14 aa 100-200. The exact sequence is proprietary.
Positive control
- Recombinant Human LGALS14 protein (ab101087) can be used as a positive control in WB. Human placenta lysate; Mouse placenta lysate; JAR cells. ICC/IF: JAR cells.
General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20ºC.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR9665
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-LGALS14 antibody [EPR9665] (ab150427)

Immunocytochemistry/ Immunofluorescence analysis of JAR (Human placenta choriocarcinoma epithelial cell) cells labeling LGALS14 with purified ab150427 at 1/100 dilution (10 µg/ml). Cells were fixed in 4% Paraformaldehyde. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Western blot - Anti-LGALS14 antibody [EPR9665] (ab150427)

Anti-LGALS14 antibody [EPR9665] (ab150427) at 1/1000 dilution (Purified) + Human placenta lysates at 15 µg

Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 16 kDa
Observed band size: 16 kDa
Flow Cytometry - Anti-LGALS14 antibody [EPR9665] (ab150427)

Flow Cytometry analysis of JAR (Human placenta choriocarcinoma epithelial cell) cells labeling LGALS14 with purified ab150427 at 1/20 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Western blot - Anti-LGALS14 antibody [EPR9665] (ab150427)

Anti-LGALS14 antibody [EPR9665] (ab150427) at 1/1000 dilution ((unpurified)) + Human placenta lysate at 10 µg

Secondary
Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 16 kDa
Western blot - Anti-LGALS14 antibody [EPR9665] (ab150427)

Anti-LGALS14 antibody [EPR9665] (ab150427) at 1/1000 dilution ((unpurified)) + Mouse placenta lysate at 10 µg

Secondary
Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 16 kDa
Immunocytochemistry/ Immunofluorescence - Anti-LGALS14 antibody [EPR9665] (ab150427)

Immunofluorescent analysis of JAR cells labelling LGALS14 with unpurified ab150427 at 1/100 dilution.
Anti-LGALS14 antibody [EPR9665] (ab150427)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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