Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LGALS14 antibody [EPR9665] (ab150427)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9665] to LGALS14
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-LGALS14 antibody [EPR9665]
    See all LGALS14 primary antibodies

  • Description

    Rabbit monoclonal [EPR9665] to LGALS14

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human LGALS14 aa 100-200. The exact sequence is proprietary.

  • Positive control

    • Recombinant Human LGALS14 protein (ab101087) can be used as a positive control in WB. Human placenta lysate; Mouse placenta lysate; JAR cells. ICC/IF: JAR cells.

  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20ºC.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR9665

  • Isotype

    IgG

  • Research areas

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-LGALS14 antibody [EPR9665] (ab150427)
    Immunocytochemistry/ Immunofluorescence - Anti-LGALS14 antibody [EPR9665] (ab150427)
    Immunocytochemistry/ Immunofluorescence analysis of JAR (Human placenta choriocarcinoma epithelial cell) cells labeling LGALS14 with purified ab150427 at 1/100 dilution (10 µg/ml). Cells were fixed in 4% Paraformaldehyde. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Western blot - Anti-LGALS14 antibody [EPR9665] (ab150427)
    Western blot - Anti-LGALS14 antibody [EPR9665] (ab150427)
    Anti-LGALS14 antibody [EPR9665] (ab150427) at 1/1000 dilution (Purified) + Human placenta lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 16 kDa
    Observed band size: 16 kDa

  • Flow Cytometry - Anti-LGALS14 antibody [EPR9665] (ab150427)
    Flow Cytometry - Anti-LGALS14 antibody [EPR9665] (ab150427)
    Flow Cytometry analysis of JAR (Human placenta choriocarcinoma epithelial cell) cells labeling LGALS14 with purified ab150427 at 1/20 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
  • Western blot - Anti-LGALS14 antibody [EPR9665] (ab150427)
    Western blot - Anti-LGALS14 antibody [EPR9665] (ab150427)
    Anti-LGALS14 antibody [EPR9665] (ab150427) at 1/1000 dilution ((unpurified)) + Human placenta lysate at 10 µg

    Secondary
    Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 16 kDa

  • Western blot - Anti-LGALS14 antibody [EPR9665] (ab150427)
    Western blot - Anti-LGALS14 antibody [EPR9665] (ab150427)
    Anti-LGALS14 antibody [EPR9665] (ab150427) at 1/1000 dilution ((unpurified)) + Mouse placenta lysate at 10 µg

    Secondary
    Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 16 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-LGALS14 antibody [EPR9665] (ab150427)
    Immunocytochemistry/ Immunofluorescence - Anti-LGALS14 antibody [EPR9665] (ab150427)

    Immunofluorescent analysis of JAR cells labelling LGALS14 with unpurified ab150427 at 1/100 dilution.

  • Anti-LGALS14 antibody [EPR9665] (ab150427)
    Anti-LGALS14 antibody [EPR9665] (ab150427)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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