Anti-KAT3B / p300 antibody (ab10485)
Key features and details
- Rabbit polyclonal to KAT3B / p300
- Suitable for: IP, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-KAT3B / p300 antibody
See all KAT3B / p300 primary antibodies -
Description
Rabbit polyclonal to KAT3B / p300 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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Immunogen
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Positive control
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab10485 was affinity purified using the peptide immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-KAT3B / p300 antibody (ab10485)All lanes : Anti-KAT3B / p300 antibody (ab10485) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Exposure time: 3 seconds
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT3B / p300 antibody (ab10485)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labeling KAT3B / p300 with ab10485 at 1/1000 (1 µg/ml). Detection: DAB.
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Immunoprecipitation - Anti-KAT3B / p300 antibody (ab10485)ab10485 detecting p300 in HeLa (human epithelial cell line from cervix adenocarcinoma) cells by Immunoprecipitation .
Left-hand panel represents a coomassie-blue stained gel loaded with 10mg of HeLa cytosolic (S100) and nuclear (NE) extracts;
For the immunoprecipitation experiment (right-hand panel), ab10485 was used at a concentration of 20 µg/10 mg of HeLa whole cell lysate (lanes correspond to 5, 15 and 50 µg of lysate). The flow-through was analysed by WB using ab10485 at a concentration of 0.04 µg/ml.
The identity of the band marked as "p300" was also confirmed using mass spectrometry.
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Western blot - Anti-KAT3B / p300 antibody (ab10485) This image is courtesy of an anonymous AbreviewAll lanes : Anti-KAT3B / p300 antibody (ab10485) at 1/5000 dilution
Lane 1 : Human MCF-7 Cell Lysate
Lane 2 : MDA-MB-231 Cell Lysate
Lysates/proteins at 50000 cells per lane.
Secondary
All lanes : Anti-Rabbit IgG VHH Single Domain (HRP) (ab191866)
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 3 minutesBlocking Solution and Diluent 5% milk in TBS
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Western blot - Anti-KAT3B / p300 antibody (ab10485) This image is courtesy of an abreview by Florian HandleAll lanes : Anti-KAT3B / p300 antibody (ab10485) at 1/4000 dilution
Lane 2 : PC3 cell lysate. (parent cell line) with BSA for 30 minutes at 20°C
Lane 3 : PC3 with dox. inducible p300 shRNA, untreated with BSA for 30 minutes at 20°C
Lane 4 : PC3 with dox. inducible p300 shRNA, doxcycline treated with 60 ng/ml doxycycline for 72 hours for shRNA induction with BSA for 30 minutes at 20°C
Lysates/proteins at 38 µg per lane.
Blocking peptides at 5 % per lane.
Secondary
All lanes : IRDye 680RD Goat anti-Rabbit IgG (H + L) at 1/15000 dilution
