Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-KAT3B / p300 antibody [3G230 / NM-11] - BSA and Azide free (ab264432)

Key features and details

  • Mouse monoclonal [3G230 / NM-11] to KAT3B / p300 - BSA and Azide free
  • Suitable for: WB, IP, ICC/IF, ChIP, IHC-P
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-KAT3B / p300 antibody [3G230 / NM-11] - BSA and Azide free
    See all KAT3B / p300 primary antibodies

  • Description

    Mouse monoclonal [3G230 / NM-11] to KAT3B / p300 - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, IP, ICC/IF, ChIP, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat

  • Immunogen

    Recombinant full length protein corresponding to Human KAT3B/ p300.

  • Positive control

    • WB: HeLa and Jurkat whole cell lysates.

  • General notes

    ab264432 is the PBS only version of ab14984.

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • ChIP - Anti-KAT3B / p300 antibody [3G230 / NM-11] - BSA and Azide free (ab264432)
    ChIP - Anti-KAT3B / p300 antibody [3G230 / NM-11] - BSA and Azide free (ab264432)

    ChIP was performed on HeLa chromatin from 4 million cells using 8ug of ab14984. Positive control locus IRS2 shows enrichment over the negative control MyoD as expected.

    This data was provided by an anonymous collaborator.

  • Western blot - Anti-KAT3B / p300 antibody [3G230 / NM-11] - BSA and Azide free (ab264432)
    Western blot - Anti-KAT3B / p300 antibody [3G230 / NM-11] - BSA and Azide free (ab264432)
    All lanes : Anti-KAT3B / p300 antibody [3G230 / NM-11] - ChIP Grade (ab14984) at 5 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
    Lane 2 : Jurkat nuclear extract lysate (ab14844)

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution

    Predicted band size: 300 kDa



    This image was produced using the same antibody clone in a different formulation ab14984.

    This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab14984 overnight at 4°C. Antibody binding was detected using an anti-mouse secondary antibody conjugated to HRP, and visualised using ECL development solution.

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