Anti-HDAC2 antibody [HDAC2-62] (ab12169)
Key features and details
- Mouse monoclonal [HDAC2-62] to HDAC2
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG2b
Overview
-
Product name
Anti-HDAC2 antibody [HDAC2-62]
See all HDAC2 primary antibodies -
Description
Mouse monoclonal [HDAC2-62] to HDAC2 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
Synthetic peptide:
SGEKTDTKGTKSEQLSNP
with N-terminally added Cysteine conjugated to KLH, corresponding to amino acids 471-488 of Human HDAC2. -
Positive control
- Whole cell extracts from NIH-3T3 cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
HDAC2-62 -
Isotype
IgG2b -
Research areas
Images
-
Western Blot using Rabbit anti-HDAC2
Lane 1 : Anti-HDAC2 antibody [HDAC2-62] (ab12169) at 5 µg
Lane 2 : Anti-HDAC2 antibody [HDAC2-62] (ab12169) at 2.5 µg
Lane 3 : Negative Control at 5 µg
All lanes : HeLa whole cell extract
-
All lanes : Anti-HDAC2 antibody [HDAC2-62] (ab12169) at 0.5 µg/ml
Lane 1 : Hek293T cell Lysate
Lane 2 : HeLa cell Lysate
Lane 3 : Jurkat cell Lysate
Lane 4 : K562 cell Lysate
Lane 5 : Neuro-2a cell Lysate
Lane 6 : NIH-3T3 cell Lysate
Secondary
All lanes : Goat Anti-Mouse IgG-Peroxidase
-
Western blot - Anti-HDAC2 antibody [HDAC2-62] (ab12169) This image is courtesy of an anonymous abreview.Anti-HDAC2 antibody [HDAC2-62] (ab12169) at 1/250 dilution + Human glioblastoma cell line at 120 µg
Secondary
HRP-conjugated Goat anti-Mouse at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 55 kDa why is the actual band size different from the predicted?
Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 5 minutes