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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Acetylation

Anti-HDAC2 antibody [HDAC2-62] (ab12169)

Price and availability

351 792 ₸

Availability

Order now and get it on Wednesday March 10, 2021

Anti-HDAC2 antibody [HDAC2-62] (ab12169)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [HDAC2-62] to HDAC2
  • Suitable for: IP, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG2b

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Overview

  • Product name

    Anti-HDAC2 antibody [HDAC2-62]
    See all HDAC2 primary antibodies
  • Description

    Mouse monoclonal [HDAC2-62] to HDAC2
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide:

    SGEKTDTKGTKSEQLSNP

    with N-terminally added Cysteine conjugated to KLH, corresponding to amino acids 471-488 of Human HDAC2.
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Whole cell extracts from NIH-3T3 cells.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: 0.0268% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    HDAC2-62
  • Isotype

    IgG2b
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • Stem Cells
    • Signaling Pathways
    • Wnt
    • HDACs
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • HDACs
    • Class I
    • Cardiovascular
    • Heart
    • Hypertrophy
    • Other

Images

  • Immunoprecipitation - Anti-HDAC2 antibody [HDAC2-62] (ab12169)
    Immunoprecipitation - Anti-HDAC2 antibody [HDAC2-62] (ab12169)

    Western Blot using Rabbit anti-HDAC2



    Lane 1 : Anti-HDAC2 antibody [HDAC2-62] (ab12169) at 5 µg
    Lane 2 : Anti-HDAC2 antibody [HDAC2-62] (ab12169) at 2.5 µg
    Lane 3 : Negative Control at 5 µg

    All lanes : HeLa whole cell extract
  • Western blot - Anti-HDAC2 antibody [HDAC2-62] (ab12169)
    Western blot - Anti-HDAC2 antibody [HDAC2-62] (ab12169)
    All lanes : Anti-HDAC2 antibody [HDAC2-62] (ab12169) at 0.5 µg/ml

    Lane 1 : Hek293T cell Lysate
    Lane 2 : HeLa cell Lysate
    Lane 3 : Jurkat cell Lysate
    Lane 4 : K562 cell Lysate
    Lane 5 : Neuro-2a cell Lysate
    Lane 6 : NIH-3T3 cell Lysate

    Secondary
    All lanes : Goat Anti-Mouse IgG-Peroxidase
  • Western blot - Anti-HDAC2 antibody [HDAC2-62] (ab12169)
    Western blot - Anti-HDAC2 antibody [HDAC2-62] (ab12169) This image is courtesy of an anonymous abreview.
    Anti-HDAC2 antibody [HDAC2-62] (ab12169) at 1/250 dilution + Human glioblastoma cell line at 120 µg

    Secondary
    HRP-conjugated Goat anti-Mouse at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 55 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 5 minutes

    See Abreview

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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