Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Apo-M antibody [8F12C6B8] (ab66379)

Key features and details

  • Mouse monoclonal [8F12C6B8] to Apo-M
  • Suitable for: Flow Cyt, WB, ICC/IF
  • Reacts with: Human, African green monkey
  • Isotype: IgG1

Overview

  • Product name

    Anti-Apo-M antibody [8F12C6B8]
    See all Apo-M primary antibodies

  • Description

    Mouse monoclonal [8F12C6B8] to Apo-M

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    African green monkey
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein corresponding to Apo-M.

  • Positive control

    • ICC/IF: L-02 cells; COS-7 cells WB: Human serum

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-Apo-M antibody [8F12C6B8] (ab66379)
    Western blot - Anti-Apo-M antibody [8F12C6B8] (ab66379)
    All lanes : Anti-Apo-M antibody [8F12C6B8] (ab66379) at 1/500 dilution

    All lanes : human serum

    Predicted band size: 21 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Apo-M antibody [8F12C6B8] (ab66379)
    Immunocytochemistry/ Immunofluorescence - Anti-Apo-M antibody [8F12C6B8] (ab66379)
    Immunofluorescnece analysis of COS-7 cells using ab66739 at a dilution of 1/200 - 1/1000, showing cytomembrane localization.
  • Immunocytochemistry/ Immunofluorescence - Anti-Apo-M antibody [8F12C6B8] (ab66379)
    Immunocytochemistry/ Immunofluorescence - Anti-Apo-M antibody [8F12C6B8] (ab66379)
    Immunofluorescnece analysis of L-02 cells using ab66739 at a dilution of 1/200 - 1/1000, showing cytomembrane localization.
  • Flow Cytometry - Anti-Apo-M antibody [8F12C6B8] (ab66379)
    Flow Cytometry - Anti-Apo-M antibody [8F12C6B8] (ab66379)
    Overlay histogram showing HepG2 cells stained with ab66379 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab66379, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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