Ammonia Assay Kit - Modified Berthelot - (Colorimetric) (ab102509)
Key features and details
- Assay type: Quantitative
- Platform: Microplate reader
- Assay time: 30 min
- Sample type: Cell culture media, Other biological fluids, Plasma, Serum, Urine
- Sensitivity: 10 µM
Overview
-
Product name
Ammonia Assay Kit - Modified Berthelot - (Colorimetric)
See all Ammonia kits -
Sample type
Urine, Serum, Plasma, Other biological fluids, Cell culture media -
Assay type
Quantitative -
Sensitivity
Assay time
0h 30mProduct overview
Ammonia Assay Kit - Modified Berthelot (Colorimetric) ab102509 provides a rapid, simple, sensitive, and reliable assay to measure ammonia.
In this non-enzymatic ammonia assay protocol, ammonia is used to form indophenol, a highly colored product easily quantifiable by colorimetry (λ=670nm) using a plate reader.
The kit can detect less than 1 nmol(~10 µM) ammonia or ammonium using either a 96 well or 384 well microwell plate.
Ammonia assay protocol summary:
- add samples and standards to wells
- add reagent 1 and reagent 2 and incubate for 30 min
- analyze with a microplate readerNotes
Ammonia assay kit ab83360 is an alternative to this product, and is the most popular ammonia assay kit.
Platform
Microplate readerProperties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 100 tests Ammonia Reagent 1 Amber 1 x 8ml Ammonia Reagent 2 Clear 1 x 4ml Ammonium Chloride Standard Yellow 1 x 0.1ml -
Research areas
-
Relevance
Ammonia is an important source of nitrogen for living systems. Nitrogen is required for the synthesis of amino acids, which are the building blocks of protein. Ammonia is a metabolic product which is created through amino acid deamination. It plays an important role in both normal and abnormal animal physiology like normal animal acid/base balance.
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 100 tests Ammonia Reagent 1 Amber 1 x 8ml Ammonia Reagent 2 Clear 1 x 4ml Ammonium Chloride Standard Yellow 1 x 0.1ml -
Research areas
-
Relevance
Ammonia is an important source of nitrogen for living systems. Nitrogen is required for the synthesis of amino acids, which are the building blocks of protein. Ammonia is a metabolic product which is created through amino acid deamination. It plays an important role in both normal and abnormal animal physiology like normal animal acid/base balance.
Images
-
Functional Studies - Ammonia Colorimetric Assay Kit (Modified Berthelot Assay) (ab102509)
Standard curve: mean of duplicates (+/- SD) with background reads subtracted.
-
Functional Studies - Ammonia Colorimetric Assay Kit (Modified Berthelot Assay) (ab102509)Ammonia measured in biological fluids showing quantity (mM) in tested samples. Samples were diluted 10-1000 fold.
