Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Tau (phospho S202 + T205) antibody [EPR20390] - BSA and Azide free (ab237594)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20390] to Tau (phospho S202 + T205) - BSA and Azide free
  • Suitable for: Dot blot, IP, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Tau (phospho S202 + T205) antibody [EPR20390] - BSA and Azide free
    See all Tau primary antibodies

  • Description

    Rabbit monoclonal [EPR20390] to Tau (phospho S202 + T205) - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human brain lysate.

  • General notes

    Ab237594 is the carrier-free version of ab210703. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab237594 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Tau (phospho S202 + T205) antibody [EPR20390] - BSA and Azide free (ab237594)
    Western blot - Anti-Tau (phospho S202 + T205) antibody [EPR20390] - BSA and Azide free (ab237594)
    All lanes : Anti-Tau (phospho S202 + T205) antibody [EPR20390] (ab210703) at 1/1000 dilution

    Lane 1 : Human brain tissue lysate
    Lane 2 : Alkaline phosphatase treated human brain tissue lysate (1 hour)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/100000 dilution

    Predicted band size: 78 kDa
    Observed band size: 50-80 kDa
    why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 21932121, PMID: 20660113).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210703).

  • Immunoprecipitation - Anti-Tau (phospho S202 + T205) antibody [EPR20390] - BSA and Azide free (ab237594)
    Immunoprecipitation - Anti-Tau (phospho S202 + T205) antibody [EPR20390] - BSA and Azide free (ab237594)

    Tau (phospho S202 + T205) was immunoprecipitated from 0.35 mg of human brain lysate with ab210703 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab210703 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Human brain lysate 10 µg (Input). 
    Lane 2: ab210703 IP in human brain lysate. 
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab210703 in human brain lysate.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 8 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210703).

  • Dot Blot - Anti-Tau (phospho S202 + T205) antibody [EPR20390] - BSA and Azide free (ab237594)
    Dot Blot - Anti-Tau (phospho S202 + T205) antibody [EPR20390] - BSA and Azide free (ab237594)

    Dot blot analysis of Tau (phospho S202+ T205) labeled with ab210703 at 1/1000 dilution.

    Lane 1: Tau (phospho S202 + T205) peptide.
    Lane 2: Tau (phospho S202) peptide.
    Lane 3: Tau (phospho T205) peptide.
    Lane 4: Tau non-phospho peptide.

    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210703).

  • Anti-Tau (phospho S202 + T205) antibody [EPR20390] - BSA and Azide free (ab237594)
    Anti-Tau (phospho S202 + T205) antibody [EPR20390] - BSA and Azide free (ab237594)

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