Anti-HDAC3 antibody (ab264126)
Key features and details
- Rabbit polyclonal to HDAC3
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-HDAC3 antibody
See all HDAC3 primary antibodies -
Description
Rabbit polyclonal to HDAC3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Orangutan -
Immunogen
Synthetic peptide within Human HDAC3 aa 375-428 (C terminal). The exact sequence is proprietary.
Database link: O15379 -
Positive control
- WB: HeLa, Ramos and NIH/3T3 whole cell lysate. IHC-P: Human ovarian carcinoma tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-HDAC3 antibody (ab264126) at 0.02 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : Ramos (Human Burkitt's lymphoma cell line) whole cell lysate at 50 µg
Lane 5 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Predicted band size: 49 kDa
Exposure time: 3 minutes4-20% SDS-PAGE.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC3 antibody (ab264126)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human ovarian carcinoma tissue labeling HDAC3 with ab264126 at 1/200 dilution. Detection: DAB.
Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.