Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HDAC3 antibody [EP462Y] - BSA and Azide free (ab247459)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP462Y] to HDAC3 - BSA and Azide free
  • Suitable for: IHC-P, ICC/IF, WB, Flow Cyt (Intra)
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-HDAC3 antibody [EP462Y] - BSA and Azide free
    See all HDAC3 primary antibodies

  • Description

    Rabbit monoclonal [EP462Y] to HDAC3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC/IF, WB, Flow Cyt (Intra)more details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247459 is the carrier-free version of ab76295.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC3 antibody [EP462Y] - BSA and Azide free (ab247459)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC3 antibody [EP462Y] - BSA and Azide free (ab247459)

    This data was developed using ab76295, the same antibody clone in a different buffer formulation.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse cerebrum tissue sections labeling HDAC3 with Purified ab76295 at 1:100 dilution (1.03 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Flow Cytometry - Anti-HDAC3 antibody [EP462Y] - BSA and Azide free (ab247459)
    Flow Cytometry - Anti-HDAC3 antibody [EP462Y] - BSA and Azide free (ab247459)

    This data was developed using ab76295, the same antibody clone in a different buffer formulation.

    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling HDAC3 with Purified ab76295 at 1:20 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
  • Immunocytochemistry - Anti-HDAC3 antibody [EP462Y] - BSA and Azide free (ab247459)
    Immunocytochemistry - Anti-HDAC3 antibody [EP462Y] - BSA and Azide free (ab247459)

    This data was developed using ab76295, the same antibody clone in a different buffer formulation.

    Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling HDAC3 with Purified ab76295 at 1:50 dilution (2.1 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC3 antibody [EP462Y] - BSA and Azide free (ab247459)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC3 antibody [EP462Y] - BSA and Azide free (ab247459)
    This data was developed using ab76295, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat kidney tissue sections labeling HDAC3 with Purified ab76295 at 1:100 dilution (1.03 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC3 antibody [EP462Y] - BSA and Azide free (ab247459)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC3 antibody [EP462Y] - BSA and Azide free (ab247459)
    This data was developed using ab76295, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung carcinoma tissue sections labeling HDAC3 with Purified ab76295 at 1:100 dilution (1.03 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Western blot - Anti-HDAC3 antibody [EP462Y] - BSA and Azide free (ab247459)
    Western blot - Anti-HDAC3 antibody [EP462Y] - BSA and Azide free (ab247459)
    All lanes : Anti-HDAC3 antibody [EP462Y] (ab76295) at 1/2000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : NIH/3T3 cell lysate
    Lane 5 : PC-12 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 49 kDa
    Observed band size: 49 kDa



    This data was developed using ab76295, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-HDAC3 antibody [EP462Y] - BSA and Azide free (ab247459)
    Western blot - Anti-HDAC3 antibody [EP462Y] - BSA and Azide free (ab247459)
    Anti-HDAC3 antibody [EP462Y] (ab76295) at 1/2000 dilution + HeLa cell lysate at 10 µg

    Secondary
    goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 49 kDa
    Observed band size: 49 kDa



    This data was developed using ab76295, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC3 antibody [EP462Y] - BSA and Azide free (ab247459)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC3 antibody [EP462Y] - BSA and Azide free (ab247459)
    This data was developed using ab76295, the same antibody clone in a different buffer formulation.ab76295, at a 1/100-1/250 dilution, staining human HDAC3 in breast carcinoma, by Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
  • Anti-HDAC3 antibody [EP462Y] - BSA and Azide free (ab247459)
    Anti-HDAC3 antibody [EP462Y] - BSA and Azide free (ab247459)

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