Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ING5 antibody [EPR23930-1] - BSA and Azide free (ab277948)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23930-1] to ING5 - BSA and Azide free
  • Suitable for: ICC, WB, Flow Cyt, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ING5 antibody [EPR23930-1] - BSA and Azide free
    See all ING5 primary antibodies

  • Description

    Rabbit monoclonal [EPR23930-1] to ING5 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC
    Human
    IP
    Mouse
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293T, HeLa, A549, MCF7, MDA-MB-231 and NIH/3T3 whole cell lysates; His-tagged human ING5 recombinant protein. ICC: HEK-293T and NIH/3T3 cells. Flow cyt: HEK-293T and NIH/3T3 cells. IP: MCF7 and NIH/3T3 whole cell lysates.

  • General notes

    ab277948 is the carrier-free version of ab259904. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab277948 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-ING5 antibody [EPR23930-1] - BSA and Azide free (ab277948)
    Western blot - Anti-ING5 antibody [EPR23930-1] - BSA and Azide free (ab277948)
    All lanes : Anti-ING5 antibody [EPR23930-1] (ab259904) at 1/1000 dilution

    Lane 1 : HEK-293T (human embryonic kidney epithelial cell), whole cell lysate
    Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 3 : A549 (human lung carcinoma epithelial cell), whole cell lysate
    Lane 4 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

    Predicted band size: 28 kDa
    Observed band size: 32 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab259904, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure times: Lane1-2: 10 secondsLane3-4: 15 seconds.

  • Immunocytochemistry - Anti-ING5 antibody [EPR23930-1] - BSA and Azide free (ab277948)
    Immunocytochemistry - Anti-ING5 antibody [EPR23930-1] - BSA and Azide free (ab277948)

    This data was developed using ab259904, the same antibody clone in a different buffer formulation.

    mmunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T cells labelling ING5 with ab259904 at 1/1000 (0.482 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000  dilution (Green). Confocal image showing strong nuclear staining in HEK-293T cells. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Western blot - Anti-ING5 antibody [EPR23930-1] - BSA and Azide free (ab277948)
    Western blot - Anti-ING5 antibody [EPR23930-1] - BSA and Azide free (ab277948)
    All lanes : Anti-ING5 antibody [EPR23930-1] (ab259904) at 1/1000 dilution

    Lane 2 : MDA-MB-231 (human breast adenocarcinoma epithelial cell), whole cell lysate
    Lane 3 : C6 (rat glial tumor glial cell), whole cell lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

    Predicted band size: 28 kDa



    This data was developed using ab259904, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

     Exposure time: Lane1-2: 7.5 secondsLane3: 70 seconds

     

  • Immunocytochemistry - Anti-ING5 antibody [EPR23930-1] - BSA and Azide free (ab277948)
    Immunocytochemistry - Anti-ING5 antibody [EPR23930-1] - BSA and Azide free (ab277948)

    This data was developed using ab259904, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 cells labelling ING5 with ab259904 at 1/1000 (0.482 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing strong nuclear staining in NIH/3T3 cells. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Flow Cytometry - Anti-ING5 antibody [EPR23930-1] - BSA and Azide free (ab277948)
    Flow Cytometry - Anti-ING5 antibody [EPR23930-1] - BSA and Azide free (ab277948)

    This data was developed using ab259904, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HEK-293T (Human embryonic kidney epithelial cell) cells labelling ING5 with ab259904 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Immunoprecipitation - Anti-ING5 antibody [EPR23930-1] - BSA and Azide free (ab277948)
    Immunoprecipitation - Anti-ING5 antibody [EPR23930-1] - BSA and Azide free (ab277948)

    This data was developed using ab259904, the same antibody clone in a different buffer formulation.

    ING5 was immunoprecipitated from 0.35 mg MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate with ab259904 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259904 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate 10 ug

    Lane 2: ab259904 IP in MCF7 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab259904 in MCF7 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

     

  • Immunoprecipitation - Anti-ING5 antibody [EPR23930-1] - BSA and Azide free (ab277948)
    Immunoprecipitation - Anti-ING5 antibody [EPR23930-1] - BSA and Azide free (ab277948)

    This data was developed using ab259904, the same antibody clone in a different buffer formulation.

    ING5 was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast), whole cell lysate with ab259904 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259904 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate 10 ug

    Lane 2: ab259904 IP in NIH/3T3 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab259904 in NIH/3T3 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

     

  • Flow Cytometry - Anti-ING5 antibody [EPR23930-1] - BSA and Azide free (ab277948)
    Flow Cytometry - Anti-ING5 antibody [EPR23930-1] - BSA and Azide free (ab277948)

    This data was developed using ab259904, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling ING5 with ab259904 at 1/500 dilution (0.1ug)(Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Western blot - Anti-ING5 antibody [EPR23930-1] - BSA and Azide free (ab277948)
    Western blot - Anti-ING5 antibody [EPR23930-1] - BSA and Azide free (ab277948)
    All lanes : Anti-ING5 antibody [EPR23930-1] (ab259904) at 1/1000 dilution

    Lane 1 : His-tagged human ING5 recombinant protein, 20 ng
    Lane 2 : His-tagged human ING4 recombinant protein, 20 ng

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

    Predicted band size: 28 kDa



    This data was developed using ab259904, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure times: Lane1: 8 seconds; Lane2: 3 minutes.

     

  • Anti-ING5 antibody [EPR23930-1] - BSA and Azide free (ab277948)
    Anti-ING5 antibody [EPR23930-1] - BSA and Azide free (ab277948)

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