Anti-KAT3B / p300 antibody [3G230 / NM-11] - ChIP Grade (ab14984)
![Anti-KAT3B / p300 antibody [3G230 / NM-11] - ChIP Grade (ab14984)](https://www.abcam.com/ps/products/14/ab14984/Images/ab14984-295119-anti-kat3b-p300-antibody-3g230-nm-11-chip-grade-chip.jpg "Anti-KAT3B / p300 antibody [3G230 / NM-11] - ChIP Grade (ab14984)")
Key features and details
- Mouse monoclonal [3G230 / NM-11] to KAT3B / p300 - ChIP Grade
- Suitable for: WB, ChIP
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-KAT3B / p300 antibody [3G230 / NM-11] - ChIP Grade
See all KAT3B / p300 primary antibodies -
Description
Mouse monoclonal [3G230 / NM-11] to KAT3B / p300 - ChIP Grade -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ChIP HumanWB Human
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Immunogen
Recombinant full length protein corresponding to Human KAT3B/ p300.
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Positive control
- WB: HeLa and Jurkat whole cell lysates.
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General notes
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
3G230 / NM-11 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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ChIP - Anti-KAT3B / p300 antibody [3G230 / NM-11] - ChIP Grade (ab14984) Image from Liu Y et al., J Biol Chem. 2012;287(49):41469-80. Fig 5(B).; doi: 10.1074/jbc.M112.367847.
The recruitment of p300 to the genes that exhibited changes in H3K56ac was analyzed using ChIP analysis, with IL-8 as a positive control and Mef2c, GATA4, and Nkx2.5 as negative controls.
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![ChIP - Anti-KAT3B / p300 antibody [3G230 / NM-11] - ChIP Grade (ab14984)](https://www.abcam.com/ps/products/14/ab14984/Images/ab14984-22242-anti-kat3b-p300-antibody-3g230-nm-11-chip-grade-chip.jpg)
ChIP - Anti-KAT3B / p300 antibody [3G230 / NM-11] - ChIP Grade (ab14984)ChIP was performed on HeLa chromatin from 4 million cells using 8ug of ab14984. Positive control locus IRS2 shows enrichment over the negative control MyoD as expected.
This data was provided by an anonymous collaborator.
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![ChIP - Anti-KAT3B / p300 antibody [3G230 / NM-11] - ChIP Grade (ab14984)](https://www.abcam.com/ps/products/14/ab14984/Images/ab14984-295118-anti-kat3b-p300-antibody-3g230-nm-11-chip-grade-chip.jpg)
ChIP - Anti-KAT3B / p300 antibody [3G230 / NM-11] - ChIP Grade (ab14984) Image from Ni S et al., Oncotarget. 2017;8(5):8010-8021. Fig 5C.; doi: 10.18632/oncotarget.14037.CBX7 promotes p300-PTEN promoter binding and Histone H3 acetylation in pancreatic cancer cells. CTRL, CBX7, and CBX7i Panc-1 cells were subjected to CHIP assay to determine the enrichment of p300 and acetylated Histone H3 on PTEN promoter. (*,# P
ChIP assay was performed using a ChIP qPCR Assay Kit. The bound PTEN promoter sequence was verified using RT-PCR (Real-Time polymerase chain reaction) assay with DNA fragments immunoprecipitated by anti-p300 antibodies (ab14984), anti-acetyl-histone H3, or control rabbit IgG. The DNA fragments were amplified by quantitative RT-PCR. The RT-PCR primers are 5′-CGG GCG GTG ATG TGG C-3′ and 5′-GCC TCA CAG CGG CTC AAC TCT-3′.
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![Western blot - Anti-KAT3B / p300 antibody [3G230 / NM-11] - ChIP Grade (ab14984)](https://www.abcam.com/ps/products/14/ab14984/Images/ab14984-22241-anti-kat3b-p300-antibody-3g230-nm-11-chip-grade-western-blot.jpg)
Western blot - Anti-KAT3B / p300 antibody [3G230 / NM-11] - ChIP Grade (ab14984)All lanes : Anti-KAT3B / p300 antibody [3G230 / NM-11] - ChIP Grade (ab14984) at 5 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 2 : Jurkat nuclear extract lysate (ab14844)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 300 kDa
Observed band size: 300 kDa
Additional bands at: 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutesThis blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab14984 overnight at 4°C. Antibody binding was detected using an anti-mouse secondary antibody conjugated to HRP, and visualised using ECL development solution.
