Anti-Human IgG Fab fragment antibody [4A11] (ab771)
![Anti-Human IgG Fab fragment antibody [4A11] (ab771)](https://www.abcam.com/ps/products/0/ab771/Images/ab771-278860-anti-human-igg-fab-fragment-antibody-4a11-flow-cytometry.jpg "Anti-Human IgG Fab fragment antibody [4A11] (ab771)")
Key features and details
- Mouse monoclonal [4A11] to Human IgG Fab fragment
- Suitable for: Flow Cyt, WB
- Reacts with: Cow, Human
- Isotype: IgG2b
Overview
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Product name
Anti-Human IgG Fab fragment antibody [4A11] -
Description
Mouse monoclonal [4A11] to Human IgG Fab fragment -
Host species
Mouse -
Specificity
This antibody reacts with Fab fragment of human IgG. Specificity was confirmed by western blotting analysis of purified Fab fragment, of human IgG, under reducing conditions. -
Tested applications
Suitable for: Flow Cyt, WBmore details -
Species reactivity
Reacts with: Cow, Human -
Immunogen
Full length native protein (purified) (Human).
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Positive control
- WB: Normal human immunoglobulins. Flow Cyt: Human peripheral blood cells.
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General notes
This product was changed from ascites to tissue culture supernatant on 24th January 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purification notes
Purified by precipitation and chromatography -
Clonality
Monoclonal -
Clone number
4A11 -
Myeloma
unknown -
Isotype
IgG2b -
Light chain type
unknown -
Research areas
Images
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Flow Cytometry - Anti-Human IgG Fab fragment antibody [4A11] (ab771)Flow Cytometry analysis of human peripheral blood cells labeling Human IgG Fab fragment with Anti-Human IgG Fab fragment antibody [4A11] (ab771).
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![Western blot - Anti-Human IgG Fab fragment antibody [4A11] (ab771)](https://www.abcam.com/ps/products/0/ab771/Images/ab771-276966-anti-human-igg-fab-fragment-antibody-4a11-western-blot.jpg)
Western blot - Anti-Human IgG Fab fragment antibody [4A11] (ab771)All lanes : Anti-Human IgG Fab fragment antibody [4A11] (ab771)
Lane 1 : Normal human immunoglobulins
Lane 2 : Normal bovine immunoglobulins
Performed under reducing conditions.
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![Flow Cytometry - Anti-Human IgG Fab fragment antibody [4A11] (ab771)](https://www.abcam.com/ps/products/0/ab771/Images/ab771-276965-anti-human-igg-fab-fragment-antibody-4a11-flow-cytometry.jpg)
Flow Cytometry - Anti-Human IgG Fab fragment antibody [4A11] (ab771)Surface staining of human peripheral blood cells with ab771. An APC-conjugated goat anti-mouse IgG was used as the secondary antibody.