Antibodies, Proteins, Kits and Reagents for Life Science

318 288 ₸ Product size

100 µl 318 288 ₸

Order now and get it on Tuesday March 02, 2021

Anti-RIP3 (phospho S227) antibody [EPR9627] (ab209384)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR9627] to RIP3 (phospho S227)
Suitable for: ELISA, Dot blot, WB
Reacts with: Human

Product name

Anti-RIP3 (phospho S227) antibody [EPR9627]
See all RIP3 primary antibodies
Description

Rabbit monoclonal [EPR9627] to RIP3 (phospho S227)
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Human

See all applications and species data
Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HT-29 treated with 20 ng/ml TNF alpha,100 nM Smac mimetic, and 20 mM z-VAD for 8 h and then harvested whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR9627
Isotype

IgG
Research areas

Western blot - Anti-RIP3 (phospho S227) antibody [EPR9627] (ab209384)

All lanes : Anti-RIP3 (phospho S227) antibody [EPR9627] (ab209384) at 1/1000 dilution

Lane 1 : HT-29 (Human colorectal adenocarcinoma epithelial cell) treated with 20 ng/ml TNF alpha, 100 nM Smac mimetic and 20µM VAD-FMK for 6 h cell lysate
Lane 2 : HT-29 (Human colorectal adenocarcinoma epithelial cell) cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 57 kDa
Observed band size: 70 kDa
why is the actual band size different from the predicted?

Exposure time: 100 seconds

Blocking/Diluting buffer and concentration 5% NFDM/TBST
Dot Blot - Anti-RIP3 (phospho S227) antibody [EPR9627] (ab209384)

Dot blot analysis of RIP3 (phospho S227) phospho peptide (Lane 1) and RIP3 non-phospho peptide (Lane 2) labeled using ab209384 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100,000 dilution.

Blocking and diluting buffer: 5% NFDM/TBST.

Exposure time: 10 seconds.
ELISA - Anti-RIP3 (phospho S227) antibody [EPR9627] (ab209384)

Direct ELISA antibody dose-response curve using ab209384. Antigen concentration of 1000 ng/mL and primary antibody concentration of 0-1000 ng/mL. A Alkaline Phosphatase-conjugated AffiniPure -conjugated goat anti-rabbit IgG (1/2500) was used as the secondary antibody.
Western blot - Anti-RIP3 (phospho S227) antibody [EPR9627] (ab209384)

All lanes : Anti-RIP3 (phospho S227) antibody [EPR9627] (ab209384)

Lane 1 : Untreated HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate
Lane 2 : HT-29 (Human colorectal adenocarcinoma cell line) treated with 20 ng/ml TNF alpha,100 nM Smac mimetic, and 20 uM z-VAD for 6 h and then harvested whole cell lysate

Predicted band size: 57 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.

Image provided by Xiaodong. Wang, National Institute of Biological Sciences, Beijing.
Anti-RIP3 (phospho S227) antibody [EPR9627] (ab209384)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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