Anti-RIP3 (phospho T231 + S232) antibody [EPR19403-52] (ab222320)
![Anti-RIP3 (phospho T231 + S232) antibody [EPR19403-52] (ab222320)](https://www.abcam.com/ps/products/222/ab222320/Images/ab222320-281000-anti-rip3-phospho-t231-s232-antibody-epr19403-52-western-blot.jpg "Anti-RIP3 (phospho T231 + S232) antibody [EPR19403-52] (ab222320)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19403-52] to RIP3 (phospho T231 + S232)
- Suitable for: WB, Dot blot
- Knockout validated
- Reacts with: Mouse
Overview
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Product name
Anti-RIP3 (phospho T231 + S232) antibody [EPR19403-52]
See all RIP3 primary antibodies -
Description
Rabbit monoclonal [EPR19403-52] to RIP3 (phospho T231 + S232) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: L-929 treated with 20 ng/ml TNF-a, 100 nM Smac mimetic and 20 µM z-VAD for 6 hr, whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19403-52 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RIP3 (phospho T231 + S232) antibody [EPR19403-52] (ab222320)All lanes : Anti-RIP3 (phospho T231 + S232) antibody [EPR19403-52] (ab222320) at 1/500 dilution
Lane 1 : Untreated L-929 (mouse connective tissue fibroblast cell line) whole cell lysate
Lane 2 : L-929 (mouse connective tissue fibroblast cell line) whole cell lysate treated with 10 ng/ml TNF-a and 20 µM z-VAD for 2 hours
Lane 3 : L-929 (mouse connective tissue fibroblast cell line) whole cell lysate treated with 10 ng/ml TNF-a and 20 µM z-VAD for 3.5 hours
Lane 4 : L-929 (mouse connective tissue fibroblast cell line) whole cell lysate treated with 20 ng/ml TNF-a, 100 nM Smac mimetic and 20 µM z-VAD for 6 hours
Lane 5 : L-929 (mouse connective tissue fibroblast cell line) whole cell lysate with RIP3 KO cells treated with 20 ng/ml TNF-a, 100 nM Smac mimetic and 20 µM z-VAD for 6 hours
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/5000 dilution
Predicted band size: 57 kDa
Exposure time: 60 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
Cells were lysed in 4% SDS buffer (4% SDS,10% 2-ME, 20% glycerol, 0.004% Bromophenol blue, Tris-HCl).
The images were kindly provided by our collaborator Dr. Jiahuai Han, Xiamen University.
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![Western blot - Anti-RIP3 (phospho T231 + S232) antibody [EPR19403-52] (ab222320)](https://www.abcam.com/ps/products/222/ab222320/Images/ab222320-280998-anti-rip3-phospho-t231-s232-antibody-epr19403-52-western-blot.jpg)
Western blot - Anti-RIP3 (phospho T231 + S232) antibody [EPR19403-52] (ab222320)All lanes : Anti-RIP3 (phospho T231 + S232) antibody [EPR19403-52] (ab222320) at 1/1000 dilution
Lane 1 : Untreated L-929 (mouse connective tissue fibroblast cell line) whole cell lysate
Lane 2 : L-929 (mouse connective tissue fibroblast cell line) whole cell lysate treated with 20 ng/ml TNF-a, 100 nM Smac mimetic and 20 µM z-VAD for 6 hours
Lane 3 : L-929 (mouse connective tissue fibroblast cell line) whole cell lysate treated with 20 ng/ml TNF-a, 100 nM Smac mimetic and 20 µM z-VAD for 6 hours, then treated with alkaline phosphatase for 1 hour
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 57 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
The higher MW bands in Lanes 1 and 2 are extra bands.
The lysates were prepared following the 1% SDS Hot Lysate buffer method.
For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).
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![Dot Blot - Anti-RIP3 (phospho T231 + S232) antibody [EPR19403-52] (ab222320)](https://www.abcam.com/ps/products/222/ab222320/Images/ab222320-280996-anti-rip3-phospho-t231-s232-antibody-epr19403-52-dot-blot.jpg)
Dot Blot - Anti-RIP3 (phospho T231 + S232) antibody [EPR19403-52] (ab222320)Dot blot analysis of RIP3 (phospho T231 + S232) labeled with ab222320 at 1/1000 dilution.
Lane 1: RIP3 (phospho T231 + S232) peptide (aa229-239);
Lane 2: RIP3 (phospho T231 + S232) peptide (aa223-236);
Lane 3: RIP3 (phospho T231) peptide (aa223-239);
Lane 4: RIP3 (phospho S232) peptide (aa223-239);
Lane 5: RIP3 non-phospho peptide (aa223-239).
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
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![Anti-RIP3 (phospho T231 + S232) antibody [EPR19403-52] (ab222320)](https://www.abcam.com/ps/products/222/ab222320/Images/ab222320-4-benefits-of-recombinant-antibodies.png)
Anti-RIP3 (phospho T231 + S232) antibody [EPR19403-52] (ab222320)
