Anti-RIP3 (phospho T231 + S232) antibody [2D7] (ab205421)
![Anti-RIP3 (phospho T231 + S232) antibody [2D7] (ab205421)](https://www.abcam.com/ps/products/205/ab205421/Images/ab205421-260895-anti-rip3-phospho-t231-s232-antibody-2d7-western-blot.jpg "Anti-RIP3 (phospho T231 + S232) antibody [2D7] (ab205421)")
Key features and details
- Mouse monoclonal [2D7] to RIP3 (phospho T231 + S232)
- Suitable for: ELISA, WB
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
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Product name
Anti-RIP3 (phospho T231 + S232) antibody [2D7]
See all RIP3 primary antibodies -
Description
Mouse monoclonal [2D7] to RIP3 (phospho T231 + S232) -
Host species
Mouse -
Tested applications
Suitable for: ELISA, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Mouse RIP3 (phospho T231 + S232).
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Positive control
- WB: Human and mouse pancreas tissue lysate.
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General notes
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
2D7 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-RIP3 (phospho T231 + S232) antibody [2D7] (ab205421)All lanes : Anti-RIP3 (phospho T231 + S232) antibody [2D7] (ab205421) at 1 µg/ml
Lane 1 : Human pancreas tissue lysate - total protein (ab29816) at 10 µg
Lane 2 : Pancreas (Mouse) Tissue Lysate at 10 µg
Lane 3 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
Lane 4 : Human pancreas tissue lysate - total protein (ab29816) at 10 µg with RIP3 Peptide (non-mod) at 1 µg/ml
Lane 5 : Pancreas (Mouse) Tissue Lysate at 10 µg with RIP3 peptide (non-mod) at 1 µg/ml
Lane 6 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg with RIP3 Peptide (non-mod) at 1 µg/ml
Lane 7 : Human pancreas tissue lysate - total protein (ab29816) with RIP3 phospho T231 + S232 peptide at 1 µg/ml
Lane 8 : Pancreas (Mouse) Tissue Lysate at 10 µg with RIP3 phospho T231 + S232 peptide at 1 µg/ml
Lane 9 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg with RIP3 phospho T231 + S232 peptide at 1 µg/ml
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) (ab65485) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 57 kDa
Exposure time: 30 seconds
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![ELISA - Anti-RIP3 (phospho T231 + S232) antibody [2D7] (ab205421)](https://www.abcam.com/ps/products/205/ab205421/Images/ab205421-260914-anti-rip3-phospho-t231-s232-antibody-2d7-elisa.jpg)
ELISA - Anti-RIP3 (phospho T231 + S232) antibody [2D7] (ab205421)Serially diluted ab205421 was bound to immobilised RIP3 phospho peptide (T231), RIP3 phospho peptide (S232), RIP3 dual phospho peptide (T231+S232) or RIP3 control peptide (RIP3-nP; all peptides at 1 microgram x mL-1). The antibody was detected by HRP-labeled goat anti-mouse IgG and signal was developed with TMB substrate.
