Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸

Order now and get it on Tuesday March 02, 2021

Anti-BMAL1 antibody [EPR20906-14] (ab235577)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20906-14] to BMAL1
Suitable for: IP, WB
Knockout validated
Reacts with: Mouse, Rat

Product name

Anti-BMAL1 antibody [EPR20906-14]
See all BMAL1 primary antibodies
Description

Rabbit monoclonal [EPR20906-14] to BMAL1
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Mouse

WB
Mouse

Rat

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Rat liver and brain tissue lysate; mouse testis lysate; mouse wild-type liver nuclear fraction. IP: Mouse testis lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IP	Mouse
WB	Mouse Rat

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20906-14
Isotype

IgG
Research areas

Western blot - Anti-BMAL1 antibody [EPR20906-14] (ab235577)

All lanes : Anti-BMAL1 antibody [EPR20906-14] (ab235577) at 1/1000 dilution

Lane 1 : Wild type mouse liver nuclear lysate
Lane 2 : Bmal1 knockout mouse liver nuclear lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

Predicted band size: 68 kDa
Observed band size: 75 kDa
why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Lysates were kindly provided by Dr Zhang Erquan.

Exposure time: 3 minutes.
Immunoprecipitation - Anti-BMAL1 antibody [EPR20906-14] (ab235577)

BMAL1 was immunoprecipitated from 0.35 mg Mouse testis tissue lysate 10 ug with ab235577 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab235577 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Mouse testis tissue lysate 10 ug

Lane 2: ab235577 IP in Mouse testis tissue lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab235577 in mouse testis tissue lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 minutes
Western blot - Anti-BMAL1 antibody [EPR20906-14] (ab235577)

All lanes : Anti-BMAL1 antibody [EPR20906-14] (ab235577) at 1/1000 dilution

Lane 1 : Mouse testis tissue lysate
Lane 2 : Rat brain tissue lysate
Lane 3 : Rat liver tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

Predicted band size: 68 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 minutes
Anti-BMAL1 antibody [EPR20906-14] (ab235577)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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