Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Histone H3 (di methyl R26) antibody [EPR17719] - BSA and Azide free (ab249954)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR17719] to Histone H3 (di methyl R26) - BSA and Azide free
Suitable for: PepArr, ICC, IHC-P, WB
Reacts with: Mouse, Rat, Human

Product name

Anti-Histone H3 (di methyl R26) antibody [EPR17719] - BSA and Azide free
See all Histone H3 primary antibodies
Description

Rabbit monoclonal [EPR17719] to Histone H3 (di methyl R26) - BSA and Azide free
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Mouse
PepArr	Human
WB	Mouse

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
General notes
Ab249954 is the carrier-free version of ab177284. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab249954 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR17719
Isotype

IgG

Western blot - Anti-Histone H3 (di methyl R26) antibody [EPR17719] - BSA and Azide free (ab249954)

Anti-Histone H3 (symmetric di methyl R26) antibody [EPR17719] (ab177284) at 1/2000 dilution + Human ovary lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 15 kDa
Observed band size: 15 kDa

Exposure time: 10 seconds

This data was developed using ab177284, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% BSA/TBST.
Western blot - Anti-Histone H3 (di methyl R26) antibody [EPR17719] - BSA and Azide free (ab249954)

All lanes : Anti-Histone H3 (symmetric di methyl R26) antibody [EPR17719] (ab177284) at 1/2000 dilution

Lane 1 : NTera-2/D1 (Human malignant pluripotent embryonic carcinoma) whole cell lysate
Lane 2 : HL-60 (Human promyelocytic leukemia cells) whole cell lysate
Lane 3 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 15 kDa
Observed band size: 15 kDa

Exposure time: 30 seconds

This data was developed using ab177284, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% BSA/TBST.
Western blot - Anti-Histone H3 (di methyl R26) antibody [EPR17719] - BSA and Azide free (ab249954)

All lanes : Anti-Histone H3 (symmetric di methyl R26) antibody [EPR17719] (ab177284) at 1/2000 dilution

Lane 1 : F9 (Mouse embryonic carcinoma cell line) whole cell lysate
Lane 2 : D3 (Mouse embryonic stem cell) whole cell lysate
Lane 3 : NIH/3T3 (Mouse embryonic fibroblast cells) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 15 kDa
Observed band size: 15 kDa

Exposure time: 10 seconds

This data was developed using ab177284, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% BSA/TBST.
Peptide Array - Anti-Histone H3 (di methyl R26) antibody [EPR17719] - BSA and Azide free (ab249954)

This data was developed using ab177284, the same antibody clone in a different buffer formulation.
ab177284 was tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (di methyl R26) antibody [EPR17719] - BSA and Azide free (ab249954)

This data was developed using ab177284, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling Histone H3 (symmetric di methyl R26) with ab177284 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on human stomach tissue is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (di methyl R26) antibody [EPR17719] - BSA and Azide free (ab249954)

This data was developed using ab177284, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling Histone H3 (symmetric di methyl R26) with ab177284 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on rat colon tissue is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (di methyl R26) antibody [EPR17719] - BSA and Azide free (ab249954)

This data was developed using ab177284, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling Histone H3 (symmetric di methyl R26) with ab177284 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on mouse colon tissue is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry - Anti-Histone H3 (di methyl R26) antibody [EPR17719] - BSA and Azide free (ab249954)

This data was developed using ab177284, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Histone H3 (symmetric di methyl R26) with ab177284 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on HeLa cell line. The nuclear counterstain is DAPI (blue).Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) (ab150120) secondary at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: ab177284 at 1/1000 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) (ab150120) at 1/1000 dilution.
-ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.
Immunocytochemistry - Anti-Histone H3 (di methyl R26) antibody [EPR17719] - BSA and Azide free (ab249954)

This data was developed using ab177284, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embryonic fibroblast) cells labeling Histone H3 (symmetric di methyl R26) with ab177284 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).Confocal image showing nuclear staining on NIH/3T3 cell line.The nuclear counterstain is DAPI (blue).Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) (ab150120) secondary antibody at 1/1000 dilution (red).The negative controls are as follows:--ve control 1: ab177284 at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) (ab150120) secondary antibody at 1/1000 dilution.-ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Anti-Histone H3 (di methyl R26) antibody [EPR17719] - BSA and Azide free (ab249954)

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