Recombinant human XIAP protein (ab198451)
Key features and details
- Expression system: Escherichia coli
- Purity: >= 60% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human XIAP protein
See all XIAP proteins and peptides -
Biological activity
Assay Conditions: 50 mM Tris-HCl, pH 7.4, 25 mM NaCl, 0.05% Tween-20, 0.01% BSA, 0.5 nM FAM-SMAC peptide. Incubate 30 minutes at room temperature. Fluorescence polarization is measured at exc 475 nm, em 528 nm.
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Purity
>= 60 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MHHHHHHYLGSRDHFALDRPSETHADYLLRTGQVVDISDTIYPRNPAMYS EEARLKSFQNWPDYAHLTPRELASAGLYYTGIGDQVQCFCCGGKLKNWEP CDRAWSEHRRHFPNCFFVLGRNLNIRSESDAVSSDRNFPNSTNLPRNPSM ADYEARIFTFGTWIYSVNKEQLARAGFYALGEGDKVKCFHCGGGLTDWKP SEDPWEQHAKWYPGCKYLLEQKGQEYINNIHLTHSLEECLVRTT -
Predicted molecular weight
28 kDa including tags -
Amino acids
120 to 356 -
Tags
His tag N-Terminus -
Additional sequence information
Bir2 and Bir3 domains; NM_001167.
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Preservative: 1.36% Imidazole
Constituents: 20% Glycerol (glycerin, glycerine), 0.63% Tris HCl, 0.02% Potassium chloride, 0.64% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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SDS-PAGE - Recombinant human XIAP protein (ab198451)
4-20% SDS-PAGE analysis of 1.8μg ab198451 with Coomassie staining.
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Functional Studies - Recombinant human XIAP protein (ab198451)Binding activity assay using ab198451.
