Antibodies, Proteins, Kits and Reagents for Life Science

314 937 ₸ Product size

100 µl 314 937 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Nuf2 antibody [EPR13018(B)] (ab176556)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR13018(B)] to Nuf2
Suitable for: WB, Flow Cyt, ICC/IF
Reacts with: Human

Product name

Anti-Nuf2 antibody [EPR13018(B)]
See all Nuf2 primary antibodies
Description

Rabbit monoclonal [EPR13018(B)] to Nuf2
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human Nuf2 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: Q9BZD4
Positive control
- 293T, A431, HeLa and Human fetal liver lysates; 293T cells.HEK 293 cells.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR13018(B)
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-Nuf2 antibody [EPR13018(B)] (ab176556)

ab176556 staining Nuf2 in HeLa (human cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/1000. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. ab195889 was used as a tubulin counterstain at a dilution of 1/200 and DAPI was used as a nuclear counterstain.
Flow Cytometry - Anti-Nuf2 antibody [EPR13018(B)] (ab176556)

ab176556 staining Nuf2 in the human cell line 293T (Human epithelial cell line from embryonic kidney) by flow cytometry. Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/60. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

Isoytype control: Rabbit monoclonal IgG (Black)

Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)
Western blot - Anti-Nuf2 antibody [EPR13018(B)] (ab176556)

All lanes : Anti-Nuf2 antibody [EPR13018(B)] (ab176556) at 1/2000 dilution

Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2 : A431 (Human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 54 kDa
Additional bands at: 54 kDa. We are unsure as to the identity of these extra bands.

Blocking and diluting buffer: 5% NFDM/TBST
Western blot - Anti-Nuf2 antibody [EPR13018(B)] (ab176556)

All lanes : Anti-Nuf2 antibody [EPR13018(B)] (ab176556) at 1/1000 dilution

Lane 1 : 293T cell lysate
Lane 2 : Human fetal liver lysate
Lane 3 : A431 cell lysate
Lane 4 : HeLa cell lysate

Lysates/proteins at 10 µg per lane.

Developed using the ECL technique.

Predicted band size: 54 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Nuf2 antibody [EPR13018(B)] (ab176556) This image is courtesy of an Abreview submitted by Kirk McManus

ab176556 staining Nuf2 in HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X-100 in PBS. Samples were incubated with primary antibody (1/500 in PBS) for 1 hour at 22°C. ab150081, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Counterstained with DAPI.
See Abreview
Flow Cytometry - Anti-Nuf2 antibody [EPR13018(B)] (ab176556)

Flow cytometry analysis of permeabilized 293T cells labeling Nuf2 (red), using ab176556 at a 1/10 dilution, or negative control rabbit IgG (green)
Anti-Nuf2 antibody [EPR13018(B)] (ab176556)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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