Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MED4 antibody [EPR7816] (ab129170)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7816] to MED4
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-MED4 antibody [EPR7816]
    See all MED4 primary antibodies

  • Description

    Rabbit monoclonal [EPR7816] to MED4

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human MED4 aa 150-250. The exact sequence is proprietary.

  • Positive control

    • WB: HepG2, T47D and K562 cell lysates. IHC-P: Human breast carcinoma and colon tissues. ICC/IF: HepG2 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-MED4 antibody [EPR7816] (ab129170)
    Western blot - Anti-MED4 antibody [EPR7816] (ab129170)
    All lanes : Anti-MED4 antibody [EPR7816] (ab129170) at 1/1000 dilution

    Lane 1 : HepG2 cell lysates
    Lane 2 : T47D cell lysates
    Lane 3 : K562 cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

    Predicted band size: 30 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED4 antibody [EPR7816] (ab129170)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED4 antibody [EPR7816] (ab129170)

    ab129170, at 1/100 dilution staining MED4 in paraffin-embedded Human breast carcinoma tissue, by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-MED4 antibody [EPR7816] (ab129170)
    Immunocytochemistry/ Immunofluorescence - Anti-MED4 antibody [EPR7816] (ab129170)

    Immunocytochemistry/Immunofluorescence analysis of HepG2 cells labelling MED4 with ab129170 at 1/1000. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
    Control: PBS only.
    Nuclear counter stain: DAPI.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED4 antibody [EPR7816] (ab129170)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED4 antibody [EPR7816] (ab129170)

    ab129170, at 1/100 dilution staining MED4 in paraffin-embedded Human colon tissue, by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-MED4 antibody [EPR7816] (ab129170)
    OI-RD Scanning - Anti-MED4 antibody [EPR7816] (ab129170)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-MED4 antibody [EPR7816] (ab129170)
    Anti-MED4 antibody [EPR7816] (ab129170)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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