Anti-BRAF (mutated V600E) antibody [RM8] (ab200535)
![Anti-BRAF (mutated V600E) antibody [RM8] (ab200535)](https://www.abcam.com/ps/products/200/ab200535/Images/ab200535-241027-anti-braf-mutated-v600-e-antibody-rm8-western-blot.jpg "Anti-BRAF (mutated V600E) antibody [RM8] (ab200535)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [RM8] to BRAF (mutated V600E)
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-BRAF (mutated V600E) antibody [RM8]
See all BRAF primary antibodies -
Description
Rabbit monoclonal [RM8] to BRAF (mutated V600E) -
Host species
Rabbit -
Specificity
ab200535 reacts to the B Raf V600E mutant. No cross reactivity with wild type B Raf. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
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Immunogen
Synthetic peptide corresponding to Human BRAF (mutated V600E).
Database link: P15056 -
Positive control
- Cell lysates prepared from cell lines expressing endogenous B Raf (mutated V600E); .Human melanoma and Human colon cancer tissues; WiDr cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 48% PBS, 50% Glycerol -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purified from an animal origin–free culture supernatant. -
Clonality
Monoclonal -
Clone number
RM8 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-BRAF (mutated V600E) antibody [RM8] (ab200535)All lanes : Anti-BRAF (mutated V600E) antibody [RM8] (ab200535) at 2 µg/ml
Lane 1 : Cell lysates prepared from cell lines expressing endogenous B Raf (mutated V600E)
Lane 2 : Cell lysates prepared from cell lines expressing endogenous wild-type protein
Predicted band size: 84 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF (mutated V600E) antibody [RM8] (ab200535)](https://www.abcam.com/ps/products/200/ab200535/Images/ab200535-241030-anti-braf-mutated-v600-e-antibody-rm8-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF (mutated V600E) antibody [RM8] (ab200535)Immunohistochemical analysis of formalin fixed and paraffin embedded WiDr cells labeling B Raf (mutated V600E) with ab200535 at 5 µg/mL.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF (mutated V600E) antibody [RM8] (ab200535)](https://www.abcam.com/ps/products/200/ab200535/Images/ab200535-241028-anti-braf-mutated-v600-e-antibody-rm8-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF (mutated V600E) antibody [RM8] (ab200535)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human melanoma tissue labeling B Raf with ab200535 at 5 µg/mL.
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![Anti-BRAF (mutated V600E) antibody [RM8] (ab200535)](https://www.abcam.com/ps/products/200/ab200535/Images/ab200535-6-benefits-of-recombinant-antibodies.png)
Anti-BRAF (mutated V600E) antibody [RM8] (ab200535)
