Anti-BRAF antibody (ab254565)
Key features and details
- Chicken polyclonal to BRAF
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgY
Overview
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Product name
Anti-BRAF antibody
See all BRAF primary antibodies -
Description
Chicken polyclonal to BRAF -
Host species
Chicken -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide within Human BRAF aa 330-380. The exact sequence is proprietary. 18 amino acid peptide.
Database link: P15056 -
Positive control
- WB: Human brain tissue lysate; Caco-2, Daudi, HEK-293, A549, HeLa, K562 Jurkat, SK-N-SH and NIH/3T3 cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Images
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Western blot - Anti-BRAF antibody (ab254565)All lanes : Anti-BRAF antibody (ab254565) at 0.5 µg/ml
Lane 1 : Caco-2 (human colorectal adenocarcinoma cell line) cell lysate
Lane 2 : Daudi (human Burkitt's lymphoma cell line) cell lysate
Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lane 4 : THP-1 (human monocytic leukemia cell line) cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-Chicken HRP at 1/10000 dilution
Predicted band size: 84 kDaPrimary incubation for 1 hour at RT in 5% NFDM/TBST.
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Western blot - Anti-BRAF antibody (ab254565)Lane 1 : Anti-BRAF antibody (ab254565) at 1 µg/ml
Lane 2 : Anti-BRAF antibody (ab254565) at 2 µg/ml
All lanes : Human brain tissue lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-Chicken HRP at 1/10000 dilution
Predicted band size: 84 kDaPrimary incubation for 1 hour at RT in 5% NFDM/TBST.
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Western blot - Anti-BRAF antibody (ab254565)All lanes : Anti-BRAF antibody (ab254565) at 0.5 µg/ml
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) cell lysate
Lane 3 : A549 (human lung carcinoma cell line) cell lysate
Lane 4 : Caco-2 (human colorectal adenocarcinoma cell line) cell lysate
Lane 5 : Daudi (human Burkitt's lymphoma cell line) cell lysate
Lane 6 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 7 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lane 8 : K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Lane 9 : MCF7 (human breast adenocarcinoma cell line) cell lysate
Lane 10 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 11 : SK-N-SH (human neuroblastoma cell line) cell lysate
Lane 12 : THP-1 (human monocytic leukemia cell line) cell lysate
Lane 13 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
Lane 14 : L1210 cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-Chicken HRP at 1/10000 dilution
Predicted band size: 84 kDaPrimary incubation for 1 hour at RT in 5% NFDM/TBST.
