Anti-BRAF (phospho T401) antibody [EPR2208Y] (ab68215)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2208Y] to BRAF (phospho T401)
- Suitable for: WB
- Reacts with: Rat
Overview
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Product name
Anti-BRAF (phospho T401) antibody [EPR2208Y]
See all BRAF primary antibodies -
Description
Rabbit monoclonal [EPR2208Y] to BRAF (phospho T401) -
Host species
Rabbit -
Specificity
This antibody detects B Raf phosphorylated on threonine 401. -
Tested Applications & Species
See all applications and species dataApplication Species WB Rat -
Immunogen
Synthetic peptide corresponding to Human BRAF.
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Positive control
- PC-12 cell lysates (cells untreated or treated with TPA)
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR2208Y -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-BRAF (phospho T401) antibody [EPR2208Y] (ab68215) at 1/5000 dilution
Lane 1 : PC-12 cell lysates (cells were untreated)
Lane 2 : PC-12 cell lysates (cells were treated with TPA)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 85 kDa
Observed band size: 85 kDa
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