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Neuroscience Neurotransmission Intracellular Signaling Kinases

Anti-BRAF (phospho T401) antibody [EPR2208Y] (ab68215)

Price and availability

304 886 ₸

Availability

Order now and get it on Wednesday March 10, 2021

Anti-BRAF (phospho T401) antibody [EPR2208Y] (ab68215)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2208Y] to BRAF (phospho T401)
  • Suitable for: WB
  • Reacts with: Rat

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Overview

  • Product name

    Anti-BRAF (phospho T401) antibody [EPR2208Y]
    See all BRAF primary antibodies
  • Description

    Rabbit monoclonal [EPR2208Y] to BRAF (phospho T401)
  • Host species

    Rabbit
  • Specificity

    This antibody detects B Raf phosphorylated on threonine 401.
  • Tested Applications & Species

    Application Species
    WB
    Rat
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human BRAF.

  • Positive control

    • PC-12 cell lysates (cells untreated or treated with TPA)
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR2208Y
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Kinases
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Other
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases
    • Cell Biology
    • Cell Cycle
    • Cell differentiation
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Signal transducers

Images

  • Western blot - Anti-BRAF (phospho T401) antibody [EPR2208Y] (ab68215)
    Western blot - Anti-BRAF (phospho T401) antibody [EPR2208Y] (ab68215)
    All lanes : Anti-BRAF (phospho T401) antibody [EPR2208Y] (ab68215) at 1/5000 dilution

    Lane 1 : PC-12 cell lysates (cells were untreated)
    Lane 2 : PC-12 cell lysates (cells were treated with TPA)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 85 kDa
    Observed band size: 85 kDa

  • Anti-BRAF (phospho T401) antibody [EPR2208Y] (ab68215)
    Anti-BRAF (phospho T401) antibody [EPR2208Y] (ab68215)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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