Antibodies, Proteins, Kits and Reagents for Life Science

278 083 ₸ Product size

50 tests 278 083 ₸

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FITC Anti-BRAF (mutated V600E) antibody [K21-F] (ab175637)

Key features and details

FITC Rabbit monoclonal [K21-F] to BRAF (mutated V600E)
Suitable for: Flow Cyt
Reacts with: Human
Conjugation: FITC. Ex: 493nm, Em: 528nm
Isotype: IgG

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Store In the Dark.
Storage buffer

Preservative: 0.05% Sodium azide
Constituents: 1% BSA, 0.33% Sodium phosphate

Aqueous buffer
Concentration information loading...
Purification notes

This immunoglobulin is the product of one single B-cell line from the crude anti-peptide polyclonal anti-serum. This antibody is purified using a propriety technique and offers a completely post-translationally modified and properly glycosylated antibody. This offers increased stability.
Clonality

Monoclonal
Clone number

K21-F
Isotype

IgG
Research areas

Flow Cytometry - FITC Anti-BRAF (mutated V600E) antibody [K21-F] (ab175637)

Profile of B-raf WT and B-raf, V600-E expression in human peripheral blood lymphocytes analyzed by the BD FACSCanto II.

Blood cells of healthy donor were fixed, permeabilized and stained with anti-human B-raf wt (green histogram), anti-human B-raf V-600E (red histogram) or with an isotype control (black histogram).
Flow Cytometry - FITC Anti-BRAF (mutated V600E) antibody [K21-F] (ab175637)

Profile of B-raf WT and B-raf, V600-E expression in HT29 cell line analyzed by the BD FACSCanto II.

HT29 cells were fixed, permeabilized and stained with anti-human B-raf wt (green histogram), anti-human B-raf V-600E (red histogram) or with an isotype control (black histogram).
Flow Cytometry - FITC Anti-BRAF (mutated V600E) antibody [K21-F] (ab175637)

Profile of B-raf, V600-E expression in human HT29 cell line analyzed by the BD FACSCanto II.

HT29 cells were fixed, permeabilized and stained with anti-human B-raf, V600-E FITC (blue) or with an isotype control (black).
Flow Cytometry - FITC Anti-BRAF (mutated V600E) antibody [K21-F] (ab175637)

Peripheral blood lymphocytes of control patient (healthy donor), patient with chronic lymphocytic leukemia and patient with glioblastoma were fixed, permeabilized and stained with ab175637 (red or green) or with an isotype control (black). Dilution 1:10 – 1:50.

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