Antibodies, Proteins, Kits and Reagents for Life Science

Alexa Fluor® 647 Anti-TNF Receptor II antibody [EPR1653] (ab199822)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Alexa Fluor® 647 Rabbit monoclonal [EPR1653] to TNF Receptor II
Suitable for: ICC/IF
Reacts with: Human
Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm

Product name

Alexa Fluor® 647 Anti-TNF Receptor II antibody [EPR1653]
See all TNF Receptor II primary antibodies
Description

Alexa Fluor® 647 Rabbit monoclonal [EPR1653] to TNF Receptor II
Host species

Rabbit
Conjugation

Alexa Fluor® 647. Ex: 652nm, Em: 668nm
Tested Applications & Species

Application Species
ICC/IF
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- ICC/IF: Jurkat cells
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
ICC/IF	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage buffer

pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR1653
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-TNF Receptor II antibody [EPR1653] (ab199822)

ab199822 staining TNF Receptor II in Jurkat cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab199822 at 1/50 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Alexa Fluor® 647 Anti-TNF Receptor II antibody [EPR1653] (ab199822)

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