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Anti-NK-1R antibody [EPR6836(2)] - BSA and Azide free (ab232653)

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  • Immunoprecipitation - Anti-NK-1R antibody [EPR6836(2)] - BSA and Azide free (ab232653)
    Immunoprecipitation - Anti-NK-1R antibody [EPR6836(2)] - BSA and Azide free (ab232653)

    Western blot analysis of NK-1R in K562 cell lysate immunoprecipitated using ab183713 at 1/50 dilution (Lane 1). Lane 2 : TBS instead of K562 lysates.

    Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183713).

  • Western blot - Anti-NK-1R antibody [EPR6836(2)] - BSA and Azide free (ab232653)
    Western blot - Anti-NK-1R antibody [EPR6836(2)] - BSA and Azide free (ab232653)
    All lanes : Anti-NK-1R antibody [EPR6836(2)] (ab183713) at 1/50000 dilution

    Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow ) cell lysate
    Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
    Lane 3 : THP-1 (human monocytic leukemia cell line) cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 46 kDa
    Observed band size: 48 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183713).

  • Anti-NK-1R antibody [EPR6836(2)] - BSA and Azide free (ab232653)
    Anti-NK-1R antibody [EPR6836(2)] - BSA and Azide free (ab232653)

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