Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TRAF2 antibody [EPR7064] - BSA and Azide free (ab249405)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7064] to TRAF2 - BSA and Azide free
  • Suitable for: WB, ICC, IHC-P
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-TRAF2 antibody [EPR7064] - BSA and Azide free
    See all TRAF2 primary antibodies

  • Description

    Rabbit monoclonal [EPR7064] to TRAF2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment corresponding to Human TRAF2.

  • Positive control

    • WB: HEK-293T. Molt-4, 293T, Raji and HeLa whole cell lysate (ab150035). IHC-P: Human kidney tissue ICC: HeLa cells.

  • General notes

    Ab249405 is the carrier-free version of ab167163. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab249405 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-TRAF2 antibody [EPR7064] - BSA and Azide free (ab249405)
    Western blot - Anti-TRAF2 antibody [EPR7064] - BSA and Azide free (ab249405)
    All lanes : Anti-TRAF2 antibody [EPR7064] (ab167163) at 1/1000 dilution

    Lane 1 : Wild-type HEK-293T cell lysate
    Lane 2 : TRAF2 knockout HEK-293T cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 55 kDa
    Observed band size: 55 kDa



    This data was developed using the same antibody clone in a different buffer formulation (ab167163).

    Lanes 1- 2: Merged signal (red and green). Green - ab167163 observed at 55 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

     ab167163 was shown to react with TRAF2 in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266060 (knockout cell lysate ab257759) was used. Wild-type HEK-293T and TRAF2 knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab167163 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF2 antibody [EPR7064] - BSA and Azide free (ab249405)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF2 antibody [EPR7064] - BSA and Azide free (ab249405)
    This data was developed using ab167163, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling TRAF2 with ab167163 at 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Western blot - Anti-TRAF2 antibody [EPR7064] - BSA and Azide free (ab249405)
    Western blot - Anti-TRAF2 antibody [EPR7064] - BSA and Azide free (ab249405)

    This data was developed using ab167163, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 cell lysate (20 µg)

    Lane 2: TRAF2 knockout HAP1 cell lysate (20 µg)

    Lane 3: Human skeletal muscle tissue lysate (20 µg)

    Lane 4: U20S cell lysate (20 µg)

    Lanes 1 and 2: Merged signal (red and green). Green - ab167163 observed at 58 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab167163 was shown to recognize TRAF2 when TRAF2 knockout samples were used, along with additional cross-reactive bands. Wild-type and TRAF2 knockout samples were subjected to SDS-PAGE. ab167163 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-TRAF2 antibody [EPR7064] - BSA and Azide free (ab249405)
    Western blot - Anti-TRAF2 antibody [EPR7064] - BSA and Azide free (ab249405)
    All lanes : Anti-TRAF2 antibody [EPR7064] (ab167163) at 1/1000 dilution

    Lane 1 : Molt-4 cell lysate
    Lane 2 : 293T (Human embryonic kidney epithelial cell) cell lysate
    Lane 3 : Raji cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 55 kDa



    This data was developed using ab167163, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-TRAF2 antibody [EPR7064] - BSA and Azide free (ab249405)
    Immunocytochemistry - Anti-TRAF2 antibody [EPR7064] - BSA and Azide free (ab249405)
    This data was developed using ab167163, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HeLa cells labeling TRAF2 with ab167163 at 1/100 dilution.
  • Anti-TRAF2 antibody [EPR7064] - BSA and Azide free (ab249405)
    Anti-TRAF2 antibody [EPR7064] - BSA and Azide free (ab249405)

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