Human Apolipoprotein AII ELISA Kit, Fluorescent (ab229423)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 7.2 pg/ml
- Range: 7.8 pg/ml - 4000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Fluorescent
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Apolipoprotein AII ELISA Kit, Fluorescent
See all Apolipoprotein A II/ApoA-II kits -
Detection method
Fluorescent -
Precision
Intra-assay Sample n Mean SD CV% Standard 5 2% Inter-assay Sample n Mean SD CV% Cell extract 3 10.6% -
Sample type
Cell culture supernatant, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
7.2 pg/ml -
Range
7.8 pg/ml - 4000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Urine 90.2 89.3% - 90.8% Cell culture media 125.2 122.2% - 127.8% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Apolipoprotein AII (ApoA2) in vitro CatchPoint SimpleStep ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Apolipoprotein AII (ApoA2) protein in human serum, plasma, urine, and cell culture supernatant samples.
This CatchPoint SimpleStep ELISA kit has been optimized for Molecular Devices Microplate Readers. Click here for a list of recommended Microplate Readers.
If using a Molecular Devices’ plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint SimpleStep ELISA Kits is available with all the protocol and analysis settings at www.softmaxpro.org.The CatchPoint SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPoint HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission.
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Notes
Apolipoprotein AII may stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism. ApoA2 forms a disulfide-linked homodimer. Apolipoprotein AII also forms a disulfide-linked heterodimer with APOD and interacts with HCV core protein, APOA1BP and NDRG1.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 100X Stoplight Red Substrate 1 x 120µl 10X Human Apolipoprotein AII Capture Antibody 1 x 600µl 10X Human Apolipoprotein AII Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 500X Hydrogen Peroxide (H2O2, 3%) 1 x 50µl Antibody Diluent 5BI 1 x 6ml Human Apolipoprotein AII Lyophilized Purified Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated Black 96-Well Microplate 1 unit Stoplight Red Substrate Buffer 1 x 12ml -
Research areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
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Function
May stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism. -
Tissue specificity
Plasma; synthesized in the liver and intestine. -
Sequence similarities
Belongs to the apolipoprotein A2 family. -
Post-translational
modificationsPhosphorylation sites are present in the extracelllular medium. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- APO A2
- Apo AII
- Apo-AII
see all -
Database links
- Entrez Gene: 336 Human
- Omim: 107670 Human
- SwissProt: P02652 Human
- Unigene: 237658 Human
Images
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Other - Human Apolipoprotein AII ELISA Kit, Fluorescent (ab229423)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human Apolipoprotein AII (ApoA2) standard curve in Sample Diluent NSBackground-subtracted data values (mean +/- SD) are graphed.
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Titration of human plasma-EDTA within the working range of the assayBackground-subtracted data values (mean +/- SD) are graphed.
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ApoA2 concentrations in 10 individual human serum donors. 100,000 X diluted serum samples from 10 apparently healthy male donors were measured using this kitInterpolated data values corrected for sample dilution are graphed in μg of ApoA2 per mL of serum (mean +/- SD, n=3). The mean of ApoA2 concentration of these ten individual serum samples was determined to be 209 µg/mL with a range of 143 – 225 µg/mL.
