Human Apolipoprotein AII Matched Antibody Pair Kit (ab216794)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Human
- Range: 31.25 pg/ml - 2000 pg/ml
Overview
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Product name
Human Apolipoprotein AII Matched Antibody Pair Kit
See all Apolipoprotein A II/ApoA-II kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
11.2 pg/ml -
Range
31.25 pg/ml - 2000 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
Human Apolipoprotein AII Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human Apolipoprotein AII.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab184859. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Human Apolipoprotein AII Capture Antibody 2 x 50µg 1 x 50µg Human Apolipoprotein AII Detector Antibody 2 x 12.5µg 1 x 12.5µg Human Apolipoprotein AII Lyophilized Protein 2 vials 1 vial -
Research areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
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Function
May stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism. -
Tissue specificity
Plasma; synthesized in the liver and intestine. -
Sequence similarities
Belongs to the apolipoprotein A2 family. -
Post-translational
modificationsPhosphorylation sites are present in the extracelllular medium. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- APO A2
- Apo AII
- Apo-AII
see all -
Database links
- Entrez Gene: 336 Human
- Omim: 107670 Human
- SwissProt: P02652 Human
- Unigene: 237658 Human
Images
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Human Apolipoprotein AII standard curve.
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Human Apolipoprotein AII Matched Antibody Pair Kit (ab216794)To learn more about the advantages of recombinant antibodies see here.
