By coupling the β-Gal assay to the highly sensitive luciferase enzyme lacZ activity can be measured quickly and easy down to extremely low levels of expression. The most sensitive lacZ method available.

Although chromogenic assays of β-galactosidase activity (i.e. X-Gal) have found extensive use as do the application of the fluorogenic substrates (for example FDG) detection of lacZ activity using luminescence can give even greater levels of sensitivity. The sensitive Glo-Gal™ substrate is cleaved first by the β-galactosidase enzyme and then in the presence of ATP, luciferase acts to produce red light output.

This kit is sold by Marker Gene under product code M1214.