RubyGlow™ Glo-Gal™ β-Galactosidase Assay Kit (ab253388)
Overview
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Product name
RubyGlow™ Glo-Gal™ β-Galactosidase Assay Kit -
Product overview
By coupling the β-Gal assay to the highly sensitive luciferase enzyme lacZ activity can be measured quickly and easy down to extremely low levels of expression. The most sensitive lacZ method available.
Although chromogenic assays of β-galactosidase activity (i.e. X-Gal) have found extensive use as do the application of the fluorogenic substrates (for example FDG) detection of lacZ activity using luminescence can give even greater levels of sensitivity. The sensitive Glo-Gal™ substrate is cleaved first by the β-galactosidase enzyme and then in the presence of ATP, luciferase acts to produce red light output.This kit is sold by Marker Gene under product code M1214.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Tested applications
Suitable for: Sandwich ELISAmore details
Properties
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Storage instructions
Please refer to protocols. -
Components 1 units Cell Lysis Buffer 2 x 10ml Reaction Buffer 2 x 10ml RubyGlow™ Luciferase 2 x 0.5mg Glo-Gal™ Substrate 2 x 1.5mg
