Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ATR (phospho T1989) antibody [EPR21991] - BSA and Azide free (ab237563)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21991] to ATR (phospho T1989) - BSA and Azide free
  • Suitable for: WB, Dot blot
  • Reacts with: Human

Overview

  • Product name

    Anti-ATR (phospho T1989) antibody [EPR21991] - BSA and Azide free
    See all ATR primary antibodies

  • Description

    Rabbit monoclonal [EPR21991] to ATR (phospho T1989) - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Untreated HEK-293 whole cell lysate; HEK-293 cell lysate treated with 2mM hydroxyurea for 16 hours.

  • General notes

    Ab237563 is the carrier-free version of ab223258. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab237563 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Dot Blot - Anti-ATR (phospho T1989) antibody [EPR21991] - BSA and Azide free (ab237563)
    Dot Blot - Anti-ATR (phospho T1989) antibody [EPR21991] - BSA and Azide free (ab237563)

    Dot blot analysis of ATR (phospho T1989) labeled with ab223258 at 1/1000 dilution.

    Lane 1: ATR (phospho T1989) peptide (aa1983-1992).
    Lane 2: ATR (phospho T1989) peptide (aa1986-1995).
    Lane 3: ATR non-phospho peptide.

    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223258).

  • Western blot - Anti-ATR (phospho T1989) antibody [EPR21991] - BSA and Azide free (ab237563)
    Western blot - Anti-ATR (phospho T1989) antibody [EPR21991] - BSA and Azide free (ab237563)
    All lanes : Anti-ATR (phospho T1989) antibody [EPR21991] (ab223258) at 1/1000 dilution

    Lane 1 : Untreated HEK-293 (human embryonic kidney epithelial cell) whole cell lysate
    Lane 2 : HEK-293 treated with 2mM hydroxyurea for 16 hours, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 301 kDa
    Observed band size: 301 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 2% BSA/TBST

    ATR Thr1989 phosphorylation is increased after DNA damage (PMID: 21777809).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223258).

     

  • Anti-ATR (phospho T1989) antibody [EPR21991] - BSA and Azide free (ab237563)
    Anti-ATR (phospho T1989) antibody [EPR21991] - BSA and Azide free (ab237563)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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