Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ATR (phospho S428) antibody [EPR2184] - BSA and Azide free (ab230831)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2184] to ATR (phospho S428) - BSA and Azide free
  • Suitable for: WB, IHC-P, Dot blot
  • Reacts with: Human

Overview

  • Product name

    Anti-ATR (phospho S428) antibody [EPR2184] - BSA and Azide free
    See all ATR primary antibodies

  • Description

    Rabbit monoclonal [EPR2184] to ATR (phospho S428) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Dot blotmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP

  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa cell lysate treated with Okadaic Acid + Calyculin A, HEK-293 cell lysate; Human prostate and Human testis tissues.

  • General notes

    Ab230831 is the carrier-free version of ab178407. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab230831 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

Properties

Images

  • Dot Blot - Anti-ATR (phospho S428) antibody [EPR2184] - BSA and Azide free (ab230831)
    Dot Blot - Anti-ATR (phospho S428) antibody [EPR2184] - BSA and Azide free (ab230831)

    Dot Blot analysis of Lane 1: ATR (pS428) phospho peptide and Lane 2: ATR non-phospho peptide labeling ATR (phospho S428) with ab178407 at 1/1000 dilution. 5% NFDM/TBST was used as the diluting and blocking buffer. ab97051 Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as the secondary antibody at 1/100000 dilution. Exposure time: 10 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab178407).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATR (phospho S428) antibody [EPR2184] - BSA and Azide free (ab230831)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATR (phospho S428) antibody [EPR2184] - BSA and Azide free (ab230831)

    This IHC data was generated using the same phospho anti-ATR antibody clone, EPR2184, in a different buffer formulation (cat# ab178407).

    Immunohistochemical analysis of paraffin embedded Human prostate tissue labeling ATR (phospho S428) with ab178407 at 1/50.

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATR (phospho S428) antibody [EPR2184] - BSA and Azide free (ab230831)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATR (phospho S428) antibody [EPR2184] - BSA and Azide free (ab230831)

    This IHC data was generated using the same phospho anti-ATR antibody clone, EPR2184, in a different buffer formulation (cat# ab178407).

    Immunohistochemical analysis of paraffin embedded Human testis tissue labeling ATR (phospho S428) with ab178407 at 1/50.

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Anti-ATR (phospho S428) antibody [EPR2184] - BSA and Azide free (ab230831)
    Anti-ATR (phospho S428) antibody [EPR2184] - BSA and Azide free (ab230831)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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