Anti-SMC1A (phospho S957) antibody [EPR2857Y] - BSA and Azide free (ab247395)
![Anti-SMC1A (phospho S957) antibody [EPR2857Y] - BSA and Azide free (ab247395)](https://www.abcam.com/ps/products/247/ab247395/Images/ab247395-405513-anti-smc1a-phospho-s957-antibody-epr2857y-bsa-and-azide-free-western-blot.jpg "Anti-SMC1A (phospho S957) antibody [EPR2857Y] - BSA and Azide free (ab247395)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2857Y] to SMC1A (phospho S957) - BSA and Azide free
- Suitable for: ICC, WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-SMC1A (phospho S957) antibody [EPR2857Y] - BSA and Azide free
See all SMC1A primary antibodies -
Description
Rabbit monoclonal [EPR2857Y] to SMC1A (phospho S957) - BSA and Azide free -
Host species
Rabbit -
Specificity
Detects SMC1A only when phosphorylated on serine 957.
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Tested applications
Suitable for: ICC, WB, IHC-Pmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab247395 is the carrier-free version of ab75768 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab247395 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR2857Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SMC1A (phospho S957) antibody [EPR2857Y] - BSA and Azide free (ab247395)All lanes : Anti-SMC1A (phospho S957) antibody [EPR2857Y] (ab75768) at 1/1000 dilution
Lane 1 : Hela cells lysate, untreated
Lane 2 : Hela cells lysate, treated with AP
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit at 1/2000 dilution
Predicted band size: 143 kDa
Observed band size: 160 kDa why is the actual band size different from the predicted?This data was developed using ab75768, the same antibody clone in a different buffer formulation.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC1A (phospho S957) antibody [EPR2857Y] - BSA and Azide free (ab247395)](https://www.abcam.com/ps/products/247/ab247395/Images/ab247395-2-anti-smc1a-phospho-s957-antibody-epr2857y-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC1A (phospho S957) antibody [EPR2857Y] - BSA and Azide free (ab247395)This data was developed using ab75768, the same antibody clone in a different buffer formulation.ab75768 at 1/100 dilution staining SMC1A in human breast carcinoma tissue, using a HRP/AP polymerized secondary antibody. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC1A (phospho S957) antibody [EPR2857Y] - BSA and Azide free (ab247395)](https://www.abcam.com/ps/products/247/ab247395/Images/ab247395-1-anti-smc1a-phospho-s957-antibody-epr2857y-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC1A (phospho S957) antibody [EPR2857Y] - BSA and Azide free (ab247395)This data was developed using ab75768, the same antibody clone in a different buffer formulation.ab75768 at 1/100 dilution staining SMC1A in human tonsil tissue, using a HRP/AP polymerized secondary antibody. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. -
![Anti-SMC1A (phospho S957) antibody [EPR2857Y] - BSA and Azide free (ab247395)](https://www.abcam.com/ps/products/247/ab247395/Images/ab247395-4-benefits-of-recombinant-antibodies.png)
Anti-SMC1A (phospho S957) antibody [EPR2857Y] - BSA and Azide free (ab247395)
