Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SMC1A (phospho S957) antibody [EPR2857Y] - BSA and Azide free (ab247395)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2857Y] to SMC1A (phospho S957) - BSA and Azide free
  • Suitable for: ICC, WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-SMC1A (phospho S957) antibody [EPR2857Y] - BSA and Azide free
    See all SMC1A primary antibodies

  • Description

    Rabbit monoclonal [EPR2857Y] to SMC1A (phospho S957) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    Detects SMC1A only when phosphorylated on serine 957.

  • Tested applications

    Suitable for: ICC, WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247395 is the carrier-free version of ab75768 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247395 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-SMC1A (phospho S957) antibody [EPR2857Y] - BSA and Azide free (ab247395)
    Western blot - Anti-SMC1A (phospho S957) antibody [EPR2857Y] - BSA and Azide free (ab247395)
    All lanes : Anti-SMC1A (phospho S957) antibody [EPR2857Y] (ab75768) at 1/1000 dilution

    Lane 1 : Hela cells lysate, untreated
    Lane 2 : Hela cells lysate, treated with AP

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit at 1/2000 dilution

    Predicted band size: 143 kDa
    Observed band size: 160 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab75768, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC1A (phospho S957) antibody [EPR2857Y] - BSA and Azide free (ab247395)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC1A (phospho S957) antibody [EPR2857Y] - BSA and Azide free (ab247395)
    This data was developed using ab75768, the same antibody clone in a different buffer formulation.ab75768 at 1/100 dilution staining SMC1A in human breast carcinoma tissue, using a HRP/AP polymerized secondary antibody. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC1A (phospho S957) antibody [EPR2857Y] - BSA and Azide free (ab247395)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC1A (phospho S957) antibody [EPR2857Y] - BSA and Azide free (ab247395)
    This data was developed using ab75768, the same antibody clone in a different buffer formulation.ab75768 at 1/100 dilution staining SMC1A in human tonsil tissue, using a HRP/AP polymerized secondary antibody. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
  • Anti-SMC1A (phospho S957) antibody [EPR2857Y] - BSA and Azide free (ab247395)
    Anti-SMC1A (phospho S957) antibody [EPR2857Y] - BSA and Azide free (ab247395)

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