Anti-ATR (phospho T1989) antibody [EPR21991] (ab223258)
![Anti-ATR (phospho T1989) antibody [EPR21991] (ab223258)](https://www.abcam.com/ps/products/223/ab223258/Images/ab223258-314782-anti-atr-phospho-t1989-antibody-epr21991-western-blot.jpg "Anti-ATR (phospho T1989) antibody [EPR21991] (ab223258)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21991] to ATR (phospho T1989)
- Suitable for: WB, Dot blot
- Reacts with: Human
Overview
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Product name
Anti-ATR (phospho T1989) antibody [EPR21991]
See all ATR primary antibodies -
Description
Rabbit monoclonal [EPR21991] to ATR (phospho T1989) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Untreated HEK-293 whole cell lysate; HEK-293 cell lysate treated with 2mM hydroxyurea for 16 hours.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21991 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ATR (phospho T1989) antibody [EPR21991] (ab223258)All lanes : Anti-ATR (phospho T1989) antibody [EPR21991] (ab223258) at 1/1000 dilution
Lane 1 : Untreated HEK-293 (human embryonic kidney epithelial cell) whole cell lysate
Lane 2 : HEK-293 treated with 2mM hydroxyurea for 16 hours, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 301 kDa
Observed band size: 301 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 2% BSA/TBST
ATR Thr1989 phosphorylation is increased after DNA damage (PMID: 21777809).
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![Dot Blot - Anti-ATR (phospho T1989) antibody [EPR21991] (ab223258)](https://www.abcam.com/ps/products/223/ab223258/Images/ab223258-314781-anti-atr-phospho-t1989-antibody-epr21991-dot-blot.jpg)
Dot Blot - Anti-ATR (phospho T1989) antibody [EPR21991] (ab223258)Dot blot analysis of ATR (phospho T1989) labeled with ab223258 at 1/1000 dilution.
Lane 1: ATR (phospho T1989) peptide (aa1983-1992).
Lane 2: ATR (phospho T1989) peptide (aa1986-1995).
Lane 3: ATR non-phospho peptide.Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 10 seconds.
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![Anti-ATR (phospho T1989) antibody [EPR21991] (ab223258)](https://www.abcam.com/ps/products/223/ab223258/Images/ab223258-3-benefits-of-recombinant-antibodies.png)
Anti-ATR (phospho T1989) antibody [EPR21991] (ab223258)
