Anti-ATR (phospho S428) antibody [EPR2184] (ab178407)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2184] to ATR (phospho S428)
- Suitable for: Dot blot, IHC-P, WB
- Reacts with: Human
Overview
-
Product name
Anti-ATR (phospho S428) antibody [EPR2184]
See all ATR primary antibodies -
Description
Rabbit monoclonal [EPR2184] to ATR (phospho S428) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human -
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ATR aa 400-500 (phospho S428) (Cysteine residue). The exact sequence is proprietary.
Database link: Q13535 -
Positive control
- HeLa cell lysate treated with Okadaic Acid + Calyculin A, HEK-293 cell lysate; Human prostate and Human testis tissues.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2184 -
Isotype
IgG -
Research areas
Images
-
All lanes : Anti-ATR (phospho S428) antibody [EPR2184] (ab178407) at 1/1000 dilution
Lane 1 : Untreated HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysates
Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) treated with UV at 60J/m2 for 2 hours. whole cell lysates
Lane 3 : HEK-293 (Human epithelial cell line from embryonic kidney) treated with UV at 60J/m2 for 2 hours. whole cell lysates. Then the membrane was incubated with phosphatase.
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 300 kDa
Observed band size: 300 kDa
Exposure time: 3 minutesBlocking/Diluting buffer 5% NFDM/TBST
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATR (phospho S428) antibody [EPR2184] (ab178407)
Immunohistochemical analysis of paraffin embedded Human prostate tissue labeling ATR (phospho S428) with ab178407 at 1/50.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
All lanes : Anti-ATR (phospho S428) antibody [EPR2184] (ab178407) at 1/1000 dilution
Lane 1 : HeLa cell lysate untreated
Lane 2 : HeLa cell lysate treated with Okadaic Acid + Calyculin A
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 300 kDa
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATR (phospho S428) antibody [EPR2184] (ab178407)
Immunohistochemical analysis of paraffin embedded Human testis tissue labeling ATR (phospho S428) with ab178407 at 1/50.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
Dot Blot analysis of Lane 1: ATR (pS428) phospho peptide and Lane 2: ATR non-phospho peptide labeling ATR (phospho S428) with ab178407 at 1/1000 dilution. 5% NFDM/TBST was used as the diluting and blocking buffer. ab97051 Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as the secondary antibody at 1/100000 dilution. Exposure time: 10 seconds.
-