Anti-ATR antibody (ab2905)
Key features and details
- Rabbit polyclonal to ATR
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ATR antibody
See all ATR primary antibodies -
Description
Rabbit polyclonal to ATR -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Fusion protein, corresponding to amino acids 400-460 of Human ATR.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ATR antibody (ab2905)Western blot on ATR in K562 whole cell lysate using ab2905.
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Western blot - Anti-ATR antibody (ab2905)All lanes : Anti-ATR antibody (ab2905) at 1/1000 dilution
Lane 1 : MCF10A breast epithelial cells, grown in normoxic conditions (20% O2). Cells were lysed with NP40.
Lane 2 : MCF10A Breast Epithelial cells grown in hypoxic. conditions (5% O2). Cells were lysed with NP40.
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti Rabbit (HRP) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 309 kDa
Observed band size: 300 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
Blocking: Carried out using non fat dry milk for 1 hr at room temperature.
This image is an edited version of an image submitted courtesy of an Abreview by Francisco Ramirez-Valle submitted on 15th November 2005. We do not have any further information relating to this image.
