Anti-ICAD antibody (ab232957)
Key features and details
- Rabbit polyclonal to ICAD
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-ICAD antibody
See all ICAD primary antibodies -
Description
Rabbit polyclonal to ICAD -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment (His-T7-tag) corresponding to Human ICAD aa 50 to the C-terminus. Expressed in E.coli. N-terminal tags.
Database link: O00273-1 -
Positive control
- WB: Recombinant human ICAD protein; Human lung lysate; HeLa, MCF7 and HEK-293T cell lysates. IHC-P: Human stomach, kidney and breast cancer tissue.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-ICAD antibody (ab232957)All lanes : Anti-ICAD antibody (ab232957) at 3 µg/ml
Lane 1 : Human lung lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : MCF7 (human breast adenocarcinoma cell line) cell lysate
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Secondary
All lanes : HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 37 kDa
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAD antibody (ab232957)Formalin-fixed, paraffin-embedded human stomach tissue stained for ICAD using ab232957 at 10 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAD antibody (ab232957)Formalin-fixed, paraffin-embedded human breast cancer tissue stained for ICAD using ab232957 at 10 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAD antibody (ab232957)Formalin-fixed, paraffin-embedded human kidney tissue stained for ICAD using ab232957 at 10 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
-
Western blot - Anti-ICAD antibody (ab232957)Anti-ICAD antibody (ab232957) at 3 µg + Recombinant human ICAD protein
Secondary
HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 37 kDa
