Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human Proinsulin ELISA Kit (ab242235)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 0.75 pmol/L
  • Range: 4.99 pmol/L - 319.5 pmol/L
  • Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human Proinsulin ELISA Kit
    See all Insulin + Proinsulin kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    protein 5 5.2%
    Inter-assay
    Sample n Mean SD CV%
    protein 3 5.6%

  • Sample type

    Serum, Hep Plasma, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    0.75 pmol/L

  • Range

    4.99 pmol/L - 319.5 pmol/L

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 107 102% - 114%
    Hep Plasma 98 95% - 104%
    EDTA Plasma 108 102% - 116%
    Cit plasma 95 92% - 102%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human Proinsulin ELISA Kit (ab242235) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Proinsulin protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Proinsulin with 7 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Proinsulin is the precursor of insulin, an essential and primary hormone involved in glucose metabolism. Proinsulin is synthesized in the beta cells of the islets of Langerhans in the pancreas, where it is processed in the ER into active, mature insulin. Active, mature insulin is stored in secretory granules. Rising blood glucose levels lead to the release of active, mature insulin into the blood stream. Proinsulin levels in the blood are elevated with age, in type II diabetes, and during beta cell dysregulation.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Images

  • Other - Human Proinsulin ELISA Kit (ab242235)
    Other - Human Proinsulin ELISA Kit (ab242235)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Human Proinsulin Standard Curve Comparison.
    Human Proinsulin Standard Curve Comparison.
    Standard Curve comparison between human Proinsulin SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
  • Example of human Proinsulin standard curve in Sample Diluent NS.
    Example of human Proinsulin standard curve in Sample Diluent NS.

    The Proinsulin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • Serum from ten individual healthy human female donors, nine individual healthy human male donors, and three individual male donors with type 2 diabetes were measured in duplicate.
    Serum from ten individual healthy human female donors, nine individual healthy human male donors, and three individual male donors with type 2 diabetes were measured in duplicate.

    Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Only those samples that showed detectable signal are shown above.

  • Sandwich ELISA - Human Proinsulin ELISA Kit (ab242235)
    Sandwich ELISA - Human Proinsulin ELISA Kit (ab242235)
    To learn more about the advantages of recombinant antibodies see here.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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