Human Proinsulin ELISA Kit (ab242235)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 0.75 pmol/L
- Range: 4.99 pmol/L - 319.5 pmol/L
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Proinsulin ELISA Kit
See all Insulin + Proinsulin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% protein 5 5.2% Inter-assay Sample n Mean SD CV% protein 3 5.6% -
Sample type
Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.75 pmol/L -
Range
4.99 pmol/L - 319.5 pmol/L -
Recovery
Sample specific recovery Sample type Average % Range Serum 107 102% - 114% Hep Plasma 98 95% - 104% EDTA Plasma 108 102% - 116% Cit plasma 95 92% - 102% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Proinsulin ELISA Kit (ab242235) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Proinsulin protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Proinsulin with 7 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Proinsulin is the precursor of insulin, an essential and primary hormone involved in glucose metabolism. Proinsulin is synthesized in the beta cells of the islets of Langerhans in the pancreas, where it is processed in the ER into active, mature insulin. Active, mature insulin is stored in secretory granules. Rising blood glucose levels lead to the release of active, mature insulin into the blood stream. Proinsulin levels in the blood are elevated with age, in type II diabetes, and during beta cell dysregulation.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Proinsulin Capture Antibody 1 x 600µl 10X Human Proinsulin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human Proinsulin Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Relevance
Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Defects in insulin are the cause of familial hyperproinsulinemia. -
Cellular localization
Secreted -
Alternative names
- ILPR
- ins
- Insulin
see all -
Database links
- Entrez Gene: 3630 Human
- Omim: 176730 Human
- SwissProt: P01308 Human
Images
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Other - Human Proinsulin ELISA Kit (ab242235)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human Proinsulin Standard Curve Comparison.Standard Curve comparison between human Proinsulin SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity. -
Example of human Proinsulin standard curve in Sample Diluent NS.The Proinsulin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Serum from ten individual healthy human female donors, nine individual healthy human male donors, and three individual male donors with type 2 diabetes were measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Only those samples that showed detectable signal are shown above.
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Sandwich ELISA - Human Proinsulin ELISA Kit (ab242235)To learn more about the advantages of recombinant antibodies see here.
