Anti-PHF23 antibody (ab264292)
Key features and details
- Rabbit polyclonal to PHF23
- Suitable for: IP, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PHF23 antibody
See all PHF23 primary antibodies -
Description
Rabbit polyclonal to PHF23 -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow
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Immunogen
Synthetic peptide within Human PHF23 aa 353-403. The exact sequence is proprietary. NP_077273.2
Database link: Q9BUL5 -
Positive control
- IHC-P: Human prostate carcinoma. WB: HeLa whole cell lysate. IP: HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PHF23 antibody (ab264292)All lanes : Anti-PHF23 antibody (ab264292) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Exposure time: 30 seconds
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Immunoprecipitation - Anti-PHF23 antibody (ab264292)PHF23 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264292 at 10 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab264292 at 0.4 µg/ml.
Lane 1: ab264292 IP in HeLa whole cell lysate.
Lane 2: Control IgG.Exposure time: 30 secs.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF23 antibody (ab264292)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human prostate carcinoma tissue labeling PHF23 with ab264292 at 1/1,000 dilution. Detection: DAB.
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
