Anti-Calumenin antibody (ab241316)
Key features and details
- Rabbit polyclonal to Calumenin
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Calumenin antibody
See all Calumenin primary antibodies -
Description
Rabbit polyclonal to Calumenin -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP Human
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Immunogen
Synthetic peptide within Human Calumenin aa 200-250. The exact sequence is proprietary.
Database link: O43852 -
Positive control
- IP: HEK-293T whole cell lysate.
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General notes
ab241316 has not performed satisfactorily when used for WB of Calumenin in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of Calumenin.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab241316 was affinity purified using an epitope specific to Calumenin immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-Calumenin antibody (ab241316)
Calumenin was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241316 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab241316 at 1 µg/ml.
Lane 1: ab241316 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
Lysate prepared using NETN lysis buffer.
